Whole genome sequencing of fecal samples as a tool for the diagnosis and genetic characterization of norovirus

Highlights • Whole genome sequencing was performed on fecal samples from patients with norovirus. • Complete norovirus sequences were obtained from all clinical samples. • Whole genome sequencing permitted characterization of different norovirus genotypes. • WGS directly on clinical samples benefits...

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Bibliographic Details
Published in:	Journal of clinical virology Vol. 72; pp. 122 - 125
Main Authors:	Bavelaar, Herjan H.J, Rahamat-Langendoen, Janette, Niesters, Hubert G.M, Zoll, Jan, Melchers, Willem J.G
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-11-2015
Subjects:	Allergy and Immunology Caliciviridae Infections - diagnosis Caliciviridae Infections - virology Feces - virology Gastroenteritis - diagnosis Gastroenteritis - virology Genome, Viral Genotype High-Throughput Nucleotide Sequencing Humans Infectious Disease Molecular Typing Next generation sequencing Norovirus Norovirus - classification Norovirus - genetics Norovirus - isolation & purification Outbreak management Phylogeny RNA, Viral - genetics RNA, Viral - isolation & purification Sequence Analysis, DNA Specimen Handling - methods Next generation sequencing Outbreak management Norovirus
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Summary:	Highlights • Whole genome sequencing was performed on fecal samples from patients with norovirus. • Complete norovirus sequences were obtained from all clinical samples. • Whole genome sequencing permitted characterization of different norovirus genotypes. • WGS directly on clinical samples benefits patient management and infection control.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2015.10.003