Validation of a new method to measure hydrogen peroxide in exhaled breath condensate

Validation of a new method to measure hydrogen peroxide in exhaled breath condensate

Inflammatory processes in the lung can or will elicit oxidative stress. The degree of oxidative stress can be determined by measuring hydrogen peroxide (H 2O 2) concentration in exhaled breath condensate (EBC) but the methods to measure H 2O 2 are all rather time consuming and only reliable and/or a...

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Bibliographic Details
Published in:Respiratory medicine Vol. 99; no. 9; pp. 1132 - 1137
Main Authors: Gerritsen, Wim B., Zanen, Pieter, Bauwens, Anja A., van den Bosch, Jules M., Haas, Fred J.
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 01-09-2005
Elsevier
Elsevier Limited
Subjects:
Adult
Air pollution
Bias
Biological and medical sciences
Biomarkers - analysis
Biosensing Techniques
Biosensor
Breath Tests - instrumentation
Breath Tests - methods
Calibration
Exhalation
Exhaled breath condensate
Female
Humans
Hydrogen peroxide
Hydrogen Peroxide - analysis
Male
Medical sciences
Methods
Middle Aged
Oxidative Stress
Pneumology
Pulmonary Disease, Chronic Obstructive - diagnosis
Reproducibility of Results
Smoking
Smoking - metabolism
Spectrometry, Fluorescence
Studies
Germany
Exhaled breath condensate
Biosensor
Hydrogen peroxide
Validation
Expired air
Method
Technique
Respiration
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Summary:Inflammatory processes in the lung can or will elicit oxidative stress. The degree of oxidative stress can be determined by measuring hydrogen peroxide (H 2O 2) concentration in exhaled breath condensate (EBC) but the methods to measure H 2O 2 are all rather time consuming and only reliable and/or accurate in the hand of skilled technicians in a dedicated laboratory. We tested a new commercial device (Ecocheck™), developed to offer a less time-consuming method to measure H 2O 2. We validated this new method according the NCCLS EP10-A2 protocol. The validation shows that the imprecision in the low range is high (28.4%) and declines with higher concentrations of H 2O 2 (up to 6.6%). The Ecocheck™ is “an easy to use” measuring device for routine measurements getting quick results without the need for skilled technicians to determine H 2O 2 concentrations.
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ISSN:0954-6111
1532-3064
DOI:10.1016/j.rmed.2005.02.020