The production of mycobacterial antigens by homogeneous culture in a fermentor

Mycobacterium bovis strain BCG, substrain 1173P2, has been grown in homogeneous culture in classical synthetic Sauton medium without supplementary ingredients. The culture conditions are described. The protein release in the culture medium and the tuberculin yield after 2% trichloroacetic acid preci...

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Bibliographic Details
Published in:	Journal of biological standardization Vol. 16; no. 4; p. 259
Main Authors:	Nyabenda, J, Bautens, E, Borremans, M, Verhofstadt, R, Weckx, M, Marchal, A
Format:	Journal Article
Language:	English
Published:	England 1988
Subjects:	Animals Antigens, Bacterial - isolation & purification Bacterial Proteins - isolation & purification Bacterial Proteins - standards Bacteriological Techniques Culture Media Fermentation Guinea Pigs Hypersensitivity, Delayed Mycobacterium bovis - growth & development Mycobacterium bovis - immunology Tuberculin - isolation & purification Tuberculin - standards
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Summary:	Mycobacterium bovis strain BCG, substrain 1173P2, has been grown in homogeneous culture in classical synthetic Sauton medium without supplementary ingredients. The culture conditions are described. The protein release in the culture medium and the tuberculin yield after 2% trichloroacetic acid precipitation were significantly improved. The antigenicity of the tuberculin has been successfully assayed on specifically sensitized guinea-pigs. It is concluded that homogeneous mycobacterium culture in a fermentor using synthetic medium is a suitable method for the large scale production of antigen.
ISSN:	0092-1157
DOI:	10.1016/0092-1157(88)90013-3