Search Results - "Baumeister, Wolfgang"

The promise and the challenges of cryo‐electron tomography by Turk, Martin, Baumeister, Wolfgang

“…Structural biologists have traditionally approached cellular complexity in a reductionist manner in which the cellular molecular components are fractionated…”
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Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate by Khoshouei, Maryam, Radjainia, Mazdak, Baumeister, Wolfgang, Danev, Radostin

“…With the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM) have changed in a profound way. These cameras are superior…”
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Cryo-electron tomography: the challenge of doing structural biology in situ by Lučič, Vladan, Rigort, Alexander, Baumeister, Wolfgang

“…Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and…”
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Cryo-EM single particle analysis with the Volta phase plate by Danev, Radostin, Baumeister, Wolfgang

“…We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is…”
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Volta potential phase plate for in-focus phase contrast transmission electron microscopy by Danev, Radostin, Buijsse, Bart, Khoshouei, Maryam, Plitzko, Jürgen M., Baumeister, Wolfgang

“…Significance Biological electron cryomicroscopy is limited by the radiation sensitivity of the samples and the consequent need to minimize exposure to the…”
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In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology by Asano, Shoh, Engel, Benjamin D., Baumeister, Wolfgang

“…Cryo-electron tomography is a powerful technique that can faithfully image the native cellular environment at nanometer resolution. Unlike many other imaging…”
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Using the Volta phase plate with defocus for cryo-EM single particle analysis by Danev, Radostin, Tegunov, Dimitry, Baumeister, Wolfgang

“…Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here,…”
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Phase separation of a yeast prion protein promotes cellular fitness by Franzmann, Titus M, Jahnel, Marcus, Pozniakovsky, Andrei, Mahamid, Julia, Holehouse, Alex S, Nüske, Elisabeth, Richter, Doris, Baumeister, Wolfgang, Grill, Stephan W, Pappu, Rohit V, Hyman, Anthony A, Alberti, Simon

“…Despite the important role of prion domains in neurodegenerative disease, their physiological function has remained enigmatic. Previous work with yeast prions…”
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Visualizing the molecular sociology at the HeLa cell nuclear periphery by Mahamid, Julia, Pfeffer, Stefan, Schaffer, Miroslava, Villa, Elizabeth, Danev, Radostin, Cuellar, Luis Kuhn, Förster, Friedrich, Hyman, Anthony A., Plitzko, Jürgen M., Baumeister, Wolfgang

“…The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo–electron tomography (cryo-ET)…”
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In Situ Structure of Neuronal C9orf72 Poly-GA Aggregates Reveals Proteasome Recruitment by Guo, Qiang, Lehmer, Carina, Martínez-Sánchez, Antonio, Rudack, Till, Beck, Florian, Hartmann, Hannelore, Pérez-Berlanga, Manuela, Frottin, Frédéric, Hipp, Mark S., Hartl, F. Ulrich, Edbauer, Dieter, Baumeister, Wolfgang, Fernández-Busnadiego, Rubén

“…Protein aggregation and dysfunction of the ubiquitin-proteasome system are hallmarks of many neurodegenerative diseases. Here, we address the elusive link…”
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Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins by Schaffer, Miroslava, Mahamid, Julia, Engel, Benjamin D., Laugks, Tim, Baumeister, Wolfgang, Plitzko, Jürgen M.

“…While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state within the cellular environment, it requires the production of…”
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In Situ Architecture and Cellular Interactions of PolyQ Inclusions by Bäuerlein, Felix J.B., Saha, Itika, Mishra, Archana, Kalemanov, Maria, Martínez-Sánchez, Antonio, Klein, Rüdiger, Dudanova, Irina, Hipp, Mark S., Hartl, F. Ulrich, Baumeister, Wolfgang, Fernández-Busnadiego, Rubén

“…Expression of many disease-related aggregation-prone proteins results in cytotoxicity and the formation of large intracellular inclusion bodies. To gain…”
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Structure of transcribing mammalian RNA polymerase II by Bernecky, Carrie, Herzog, Franz, Baumeister, Wolfgang, Plitzko, Jürgen M., Cramer, Patrick

“…The first high-resolution, cryo-electron microscopy structure of mammalian RNA polymerase II, in the form of a transcribing complex comprising DNA template and…”
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Phase-plate cryo-EM structure of a class B GPCR–G-protein complex by Liang, Yi-Lynn, Khoshouei, Maryam, Radjainia, Mazdak, Zhang, Yan, Glukhova, Alisa, Tarrasch, Jeffrey, Thal, David M., Furness, Sebastian G. B., Christopoulos, George, Coudrat, Thomas, Danev, Radostin, Baumeister, Wolfgang, Miller, Laurence J., Christopoulos, Arthur, Kobilka, Brian K., Wootten, Denise, Skiniotis, Georgios, Sexton, Patrick M.

“…Class B G-protein-coupled receptors are major targets for the treatment of chronic diseases, such as osteoporosis, diabetes and obesity. Here we report the…”
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High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2 by Zinzula, Luca, Basquin, Jerome, Bohn, Stefan, Beck, Florian, Klumpe, Sven, Pfeifer, Günter, Nagy, István, Bracher, Andreas, Hartl, F. Ulrich, Baumeister, Wolfgang

“…Unprecedented by number of casualties and socio-economic burden occurring worldwide, the coronavirus disease 2019 (Covid-19) pandemic caused by the severe…”
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A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits by Wilfling, Florian, Lee, Chia-Wei, Erdmann, Philipp S., Zheng, Yumei, Sherpa, Dawafuti, Jentsch, Stefan, Pfander, Boris, Schulman, Brenda A., Baumeister, Wolfgang

“…Autophagy eliminates cytoplasmic content selected by autophagy receptors, which link cargo to the membrane-bound autophagosomal ubiquitin-like protein…”
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Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor–Gs complex by Liang, Yi-Lynn, Khoshouei, Maryam, Glukhova, Alisa, Furness, Sebastian G. B., Zhao, Peishen, Clydesdale, Lachlan, Koole, Cassandra, Truong, Tin T., Thal, David M., Lei, Saifei, Radjainia, Mazdak, Danev, Radostin, Baumeister, Wolfgang, Wang, Ming-Wei, Miller, Laurence J., Christopoulos, Arthur, Sexton, Patrick M., Wootten, Denise

“…The structure of the glucagon-like peptide 1 receptor (GLP-1R) with its biased agonist exendin-P5 sheds light on both receptor activation and the mechanism of…”
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Structure of the human 26S proteasome at a resolution of 3.9 Å by Schweitzer, Andreas, Aufderheide, Antje, Rudack, Till, Beck, Florian, Pfeifer, Günter, Plitzko, Jürgen M., Sakata, Eri, Schulten, Klaus, Förster, Friedrich, Baumeister, Wolfgang

“…Protein degradation in eukaryotic cells is performed by the Ubiquitin-Proteasome System (UPS). The 26S proteasome holocomplex consists of a core particle (CP)…”
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Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor by Liang, Yi-Lynn, Khoshouei, Maryam, Deganutti, Giuseppe, Glukhova, Alisa, Koole, Cassandra, Peat, Thomas S., Radjainia, Mazdak, Plitzko, Jürgen M., Baumeister, Wolfgang, Miller, Laurence J., Hay, Deborah L., Christopoulos, Arthur, Reynolds, Christopher A., Wootten, Denise, Sexton, Patrick M.

“…Calcitonin gene-related peptide (CGRP) is a widely expressed neuropeptide that has a major role in sensory neurotransmission. The CGRP receptor is a…”
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Liquid-crystalline phase transitions in lipid droplets are related to cellular states and specific organelle association by Mahamid, Julia, Tegunov, Dimitry, Maiser, Andreas, Arnold, Jan, Leonhardt, Heinrich, Plitzko, Jürgen M., Baumeister, Wolfgang

“…Lipid droplets (LDs) are ubiquitous organelles comprising a central hub for cellular lipid metabolism and trafficking. This role is tightly associated with…”
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