Molecular detection of fungi of public health importance in wild animals from Southern Brazil

Summary Some animals have an important relationship with fungal infections, and searching for pathogens in animal samples may be an opportunity for eco‐epidemiological research. Since studies involving wildlife are generally restricted, using samples from road kills is an alternative. The aim of thi...

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Published in:	Mycoses Vol. 61; no. 7; pp. 455 - 463
Main Authors:	Losnak, Debora O., Rocha, Francielle R., Almeida, Barbara S., Batista, Keila Z. S., Althoff, Sérgio L., Haupt, Josiane, Ruiz, Luciana S., Anversa, Laís, Lucheis, Simone B., Paiz, Laís M., Donalisio, Maria Rita, Richini Pereira, Virginia B.
Format:	Journal Article
Language:	English
Published:	Germany Wiley Subscription Services, Inc 01-07-2018
Subjects:	Animals Animals, Wild - microbiology Aspergillus - genetics Aspergillus - isolation & purification Aspergillus penicillioides Brazil - epidemiology Cryptococcus Cryptococcus neoformans - genetics Cryptococcus spp DNA Primers DNA, Fungal - genetics Epidemiology Fish Foxes - microbiology Fungal infections Fungi Fungi - genetics Fungi - isolation & purification Fungi - pathogenicity Gene mapping Haplorhini - microbiology Histoplasma - genetics Histoplasma - isolation & purification Histoplasma capsulatum Humans Monkey Diseases - diagnosis Monkey Diseases - epidemiology Monkey Diseases - microbiology Mycoses - diagnosis Mycoses - epidemiology Mycoses - microbiology Mycoses - veterinary Paracoccidioides - genetics Paracoccidioides - isolation & purification Paracoccidioides brasiliensis pathogenic fungi Pathogens PCR Polymerase Chain Reaction Primers Public Health Raccoons - microbiology Brazil Histoplasma capsulatum Aspergillus penicillioides Cryptococcus spp pathogenic fungi PCR Paracoccidioides brasiliensis
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Summary:	Summary Some animals have an important relationship with fungal infections, and searching for pathogens in animal samples may be an opportunity for eco‐epidemiological research. Since studies involving wildlife are generally restricted, using samples from road kills is an alternative. The aim of this study was to verify whether pathogenic fungi of public health importance occur in wildlife road kills from Santa Catarina State, Brazil. Organ samples (n = 1063) from 297 animals were analysed according to Polymerase Chain Reaction (PCR) using universal primers to detect fungi in general and, subsequently, using primers specific to Paracoccidioides brasiliensis, Histoplasma capsulatum and Cryptococcus spp. There were 102 samples positive for fungal species. Eight samples were positive for P. brasiliensis, three samples were positive for Cryptococcus spp. and one sample had coinfection by these two fungi. No sample was positive for Histoplasma spp. according to the molecular detection. Genetic sequencing allowed the identification of Fungal sp. in 89 samples, Cryptococcus neoformans in two samples and Aspergillus penicillioides in three samples. This study shows the importance of wild animals in the epidemiology of fungal infections and assists in the mapping of pathogen occurrence in a region that was not previously evaluated.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0933-7407 1439-0507
DOI:	10.1111/myc.12767