Isolation and Identification of Isolate, S1 with High Biotransformation Potential of Ferulic Acid to Vanillin

Nine actinobacterial isolates were purified from the sediment sample of Kogilvai village, Warangal, Telangana, based on their capability to grow on the minimal medium with Ferulic acid (FA) as only Carbon (C) source. FA to Vanillin conversion capacity of these isolates was identified by Thin-layer c...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of pure & applied microbiology : an international research journal of microbiology Vol. 18; no. 3; pp. 1601 - 1609
Main Authors:	Bathini, Nagaraju, Esampally, Sai Krishna, Korripally, Premsagar, Sultanpuram, Vishnuvardhan Reddy, Mothe, Thirumala
Format:	Journal Article
Language:	English
Published:	Oriental Scientific Publishing Company 01-09-2024 Journal of Pure and Applied Microbiology
Subjects:	actinobacteria Dextrose ferulic acid Fructose Glucose High performance liquid chromatography molecular characterization Sediments (Geology) Sugars India
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Nine actinobacterial isolates were purified from the sediment sample of Kogilvai village, Warangal, Telangana, based on their capability to grow on the minimal medium with Ferulic acid (FA) as only Carbon (C) source. FA to Vanillin conversion capacity of these isolates was identified by Thin-layer chromatography (TLC). Biotransformation of FA to Vanillin was high by four isolates, S1, S3, O3 and O4 when compared to other five isolates (O1, O2, S2, S4 and S5) with initial pH 7 in basal medium. Among these four isolates, optimal and rapid FA to Vanillin bioconversion of 140 mg/L was shown by isolate S1 with UV-spectrophotometry. Its conversion was confirmed by High Performance Liquid Chromatography (HPLC) analysis with retention time of 2.9 min after 28hrs of incubation at 37°C with 1g/L ferulic acid in the medium with 150 rpm. Isolate S1 could utilize Lactose, Maltose, Glycerol, Fructose, Galactose, Sucrose, Dextrose, L-Arabinose, ONPG, Esculin and not other carbohydrates present in the Himedia Hicarbo kit. Molecular characterization showed that 16S rDNA gene sequence of isolate S1 was 98.27% similar to Limosilactobacillus fermentum CECT 562 with completeness of 96.7% and was identified as Limosilactobacillus sp. 16S rDNA gene sequence of isolate S1 was submitted to NCBI GenBank and its accession number was OR136396. As this isolate has high potential of FA to Vanillin biotransformation capacity, it can be further explored to be used for industrial setups for commercial exploitation.
ISSN:	0973-7510 2581-690X
DOI:	10.22207/JPAM.18.3.09