Xenogenic oogenesis of chicken ( Gallus domesticus) female primordial germ cells in germline chimeric quail ( Coturnix japonica) ovary

Xenogenic oogenesis of chicken ( Gallus domesticus) female primordial germ cells in germline chimeric quail ( Coturnix japonica) ovary

In present study, chicken primordial germ cells (PGCs) were transferred into quail embryos to investigate the development of these germ cells in quail ovary. Briefly, 2 μl of chicken embryonic blood (stage 14) or about 100 purified circulating PGCs were transferred into quail embryo. Contribution of...

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Bibliographic Details
Published in:Animal reproduction science Vol. 101; no. 3; pp. 344 - 350
Main Authors: Liu, C.H., Chang, I.K., Sasse, J., Dumatol, C.J., Basker, J.V., Wernery, U.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-10-2007
Subjects:
Animals
Cell Differentiation
Cell Movement
Cell Proliferation
Chick Embryo
Chicken
Chickens
Chimera
chimerism
Coturnix
cytogenetics
DNA
embryo (animal)
embryogenesis
Female
females
hens
in vivo studies
Interspecies chimera
Japanese quails
oocytes
Oogenesis
oogonia
ova
ova transfer
ovaries
Ovary - embryology
Ovary - physiology
Ovum - cytology
Ovum - growth & development
primordial germ cells
Primordial germ cells (PGCs)
Quail - embryology
Interspecies chimera
Oogenesis
Chicken
Primordial germ cells (PGCs)
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Summary:In present study, chicken primordial germ cells (PGCs) were transferred into quail embryos to investigate the development of these germ cells in quail ovary. Briefly, 2 μl of chicken embryonic blood (stage 14) or about 100 purified circulating PGCs were transferred into quail embryo. Contribution of chicken PGCs were detected in gonads of chimeric quail embryos (stage 28) by immunocytochemical staining of cell surface antigen SSEA-1, and by in situ hybridization (ISH) with female chicken specific DNA probe. As a result, 52.0 ± 43.2 ( n = 18) and 42.7 ± 27.3 ( n = 17) chicken PGCs were found in the gonads of chimeric quail embryo that was injected with chicken embryonic blood (stage 14) and about 100 purified circulating PGCs, respectively. Furthermore, the ovaries of 81.8% (9/11) 12 days post incubation (dpi) chimeric quail embryos were observed with a mean of 457.6 ± 237.1 female chicken PGCs-derived oogonia scattered in ovarian cortex area. In 9 out of 12 newly hatched and one week old chimeric quail chicks, on average of 2883.0 ± 1924.1 primary oocytes and 3 follicles derived from chicken PGCs were found, respectively. The present results suggest that chicken female PGCs are able to migrate, colonize, proliferate and differentiate into oogonia, primary oocytes in chimeric quail ovary.
Bibliography:http://dx.doi.org/10.1016/j.anireprosci.2006.12.021
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2006.12.021