Search Results - "Bashford, C L"

Specialized scanning ion‐conductance microscope for imaging of living cells by Korchev, Y. E., Milovanovic, M., Bashford, C. L., Bennett, D. C., Sviderskaya, E. V., Vodyanoy, I., Lab, M. J.

“…A specialized scanning ion conductance microscope (SICM) for imaging living cells has been developed from a conventional patch‐clamp apparatus, which uses a…”
Get full text

Conductance Studies on Trichotoxin_A50E and Implications for Channel Structure by Duclohier, H., Alder, G.M., Bashford, C.L., Brückner, H., Chugh, J.K., Wallace, B.A.

“…Trichotoxin_A50E is an 18-residue peptaibol whose crystal structure has recently been determined. In this study, the conductance properties of trichotoxin_A50E…”
Get full text

Understanding the tumor metabolic phenotype in the genomic era by Stubbs, M, Bashford, C L, Griffiths, J R

“…Now, at the beginning of a new century, 80 years after Warburg's Nobel prize winning discoveries, we are beginning to make sense of the underlying causes of…”
Get more information

A novel explanation for fluctuations of ion current through narrow pores by Korchev, Y. E., Bashford, C. L., Alder, G. M., Apel, P. Y., Edmonds, D. T., Lev, A. A., Nandi, K., Zima, A. V., Pasternak, C. A.

“…Fluctuation of ion current, between a high conductance and a low conductance state, through biological ion channels and pores is assumed to arise from…”
Get full text

Influence of pH on the uptake of 5-fluorouracil into isolated tumour cells by OJUGO, A. S. E, MCSHEEHY, P. M. J, STUBBS, M, ALDER, G, BASHFORD, C. L, MAXWELL, R. J, LEACH, M. O, JUDSON, I. R, GRIFFITHS, J. R

“…To investigate the possible dependence of 5-fluorouracil (5FU) uptake in tumours on the intra- (pHi) and extracellular (pHe) pH, a pH gradient (deltapH) was…”
Get full text

Rapid Switching of Ion Current in Narrow Pores: Implications for Biological Ion Channels by Lev, A. A., Korchev, Y. E., Rostovtseva, T. K., Bashford, C. L., Edmonds, Donald T., Pasternak, C. A.

“…Ions flowing through purely synthetic filters made of polyethylene terephathalate which have been etched to produce narrow pores show: (i) rapid transitions…”
Get full text

Membrane damage by hemolytic viruses, toxins, complement, and other cytotoxic agents. A common mechanism blocked by divalent cations by Bashford, C L, Alder, G M, Menestrina, G, Micklem, K J, Murphy, J J, Pasternak, C A

“…Hemolytic viruses, bacterial and animal toxins, the components of activated complement, cationic proteins, and detergents induce a sequence of permeability…”
Get full text

Thermal control of drug release by a responsive ion track membrane observed by radio tracer flow dialysis by Spohr, Reimar, Reber, Nicole, Wolf, Alexander, Alder, Glenn M., Ang, Vincent, Bashford, C.Lindsay, Pasternak, Charles A., Hideki Omichi, Yoshida, Masaru

“…The combination of a responsive hydrogel with a rigid porous supporting structure yields a membrane with high mechanical strength and high on-off-permeability…”
Get full text

Low conductance states of a single ion channel are not 'closed' by Korchev, Y E, Bashford, C L, Alder, G M, Kasianowicz, J J, Pasternak, C A

“…We have used a polymer-exclusion method to estimate the sizes of the high- and low-conductance states of Staphylococcus aureus alpha-toxin channels across…”
Get full text

Scanning ion conductance microscopy of living cells by Korchev, Y.E., Bashford, C.L., Milovanovic, M., Vodyanoy, I., Lab, M.J.

“…Currently there is a great interest in using scanning probe microscopy to study living cells. However, in most cases the contact the probe makes with the soft…”
Get full text

Triton channels are sensitive to divalent cations and protons by ROSTOVTSEVA, T. K, BASHFORD, C. L, LEV, A. A, PASTERNAK, C. A

“…Addition of Triton X-100 to planar bilayers composed of dioleoyl phosphatidyl choline, diphytanoyl phosphatidyl choline or mono-oleoyl glycerol induces single…”
Get full text

Divalent cation-sensitive pores formed by natural and synthetic melittin and by Triton X-100 by Alder, G M, Arnold, W M, Bashford, C L, Drake, A F, Pasternak, C A, Zimmermann, U

“…Leakage of ions and low-molecular-weight metabolites from Lettre cells is induced by synthetic melittin, as effectively as by melittin isolated from bee venom;…”
Get more information

Differential sensitivity of pneumolysin-induced channels to gating by divalent cations by KORCHEV, Y. E, BASHFORD, C. L, PASTERNAK, C. A

“…The induction of channels across planar lipid bilayers by purified, recombinant pneumolysin (a hemolytic protein from Streptococcus pneumoniae) has been…”
Get full text

Diffusion through narrow pores: movement of ions, water and nonelectrolytes through track-etched PETP membranes by Rostovtseva, T K, Bashford, C L, Alder, G M, Hill, G N, McGiffert, C, Apel, P Y, Lowe, G, Pasternak, C A

“…The rates at which ions (86Rb+, [3H]-choline, 36Cl), 3H2O and nonelectrolytes ([14C]-urea, [14C]-glycerol, and [14C]-sugars) equilibrate across track-etched…”
Get full text

Pore-forming toxins: experiments with S. aureus alpha-toxin, C. perfringens theta-toxin and E. coli haemolysin in lipid bilayers, liposomes and intact cells by Menestrina, G, Bashford, C L, Pasternak, C A

“…Three quite different bacterial toxins (S. aureus alpha-toxin, C. perfringens theta-toxin and E. coli haemolysin) induce the leakage of phosphorylated…”
Get more information

Staphylococcus aureus alpha-toxin-induced pores: channel-like behavior in lipid bilayers and patch clamped cells by Korchev, Y E, Alder, G M, Bakhramov, A, Bashford, C L, Joomun, B S, Sviderskaya, E V, Usherwood, P N, Pasternak, C A

“…The conductance of pores induced by Staphylococcus aureus alpha-toxin in Lettre cells has been compared to that in bilayers composed of synthetic lipids or…”
Get full text

Pore formation by S. aureus alpha-toxin in liposomes and planar lipid bilayers: effects of nonelectrolytes by Bashford, C L, Alder, G M, Fulford, L G, Korchev, Y E, Kovacs, E, MacKinnon, A, Pederzolli, C, Pasternak, C A

“…Nonelectrolytes such as polyethylene glycols (PEG) and dextrans (i) promote the association of S. aureus alpha-toxin with liposomes (shown by Coomassie…”
Get full text

Cell damage by cytolysin. Spontaneous recovery and reversible inhibition by divalent cations by Bashford, CL, Menestrina, G, Henkart, PA, Pasternak, CA

“…Cytolysin-induced membrane damage (which requires low Ca2+) has been studied 1) in E by assay of hemolysis, 2) in Lettre cells by measurement of transmembrane…”
Get full text

Ion modulation of membrane permeability: effect of cations on intact cells and on cells and phospholipid bilayers treated with pore-forming agents by BASHFORD, C. L, ALDER, G. M, GRAHAM, J. M, MENESTRINA, G, PASTERNAK, C. A

“…Leakage of ions (Na+, K+) and phosphorylated metabolites (phosphorylcholine, 2-deoxyglucose 6-phosphate) through membrane lesions in intact cells or in cells…”
Get full text

Plasma membrane potential of Lettré cells does not depend on cation gradients but on pumps by BASHFORD, C. L, PASTERNAK, C. A

“…The plasma membrane potential of Lettré cells has been determined with the optical indicator oxonol-V and found to be -57 mV at 37 degrees C (range -20 to -80…”
Get full text