Host Langerin (CD207) is a receptor for Yersinia pestis phagocytosis and promotes dissemination

Yersinia pestis is a Gram‐negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved...

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Bibliographic Details
Published in:	Immunology and cell biology Vol. 93; no. 9; pp. 815 - 824
Main Authors:	Yang, Kun, Park, Chae G, Cheong, Cheolho, Bulgheresi, Silvia, Zhang, Shusheng, Zhang, Pei, He, Yingxia, Jiang, Lingyu, Huang, Hongping, Ding, Honghui, Wu, Yiping, Wang, Shaogang, Zhang, Lin, Li, Anyi, Xia, Lianxu, Bartra, Sara S, Plano, Gregory V, Skurnik, Mikael, Klena, John D, Chen, Tie
Format:	Journal Article
Language:	English
Published:	United States Nature Publishing Group 01-10-2015 Blackwell Science Ltd
Subjects:	Animals Antigen-Presenting Cells - immunology Antigen-Presenting Cells - microbiology Antigens, CD - immunology Antigens, CD - metabolism Bacterial Adhesion - immunology Cells, Cultured CHO Cells Cricetinae Cricetulus Dendritic Cells - immunology Dendritic Cells - metabolism Flow Cytometry Host-Pathogen Interactions - immunology Humans Langerhans Cells - immunology Langerhans Cells - metabolism Lectins, C-Type - immunology Lectins, C-Type - metabolism Mannose-Binding Lectins - immunology Mannose-Binding Lectins - metabolism Mice O Antigens - immunology O Antigens - metabolism Original Phagocytosis - immunology Plague - immunology Plague - microbiology Protein Binding - immunology Receptors, Cell Surface - immunology Receptors, Cell Surface - metabolism Survival Analysis Yersinia pestis - immunology Yersinia pestis - metabolism Yersinia pestis - physiology
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Summary:	Yersinia pestis is a Gram‐negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen‐presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium‐dependent (C‐type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin‐expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin‐expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin‐expressing transfectants is inhibited by purified Langerin, a DC‐SIGN (DC‐specific intercellular adhesion molecule 3 grabbing nonintegrin)‐like molecule, an anti‐CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis. Therefore, Langerin‐mediated binding of Y. pestis to APCs may promote its dissemination and infection.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0818-9641 1440-1711
DOI:	10.1038/icb.2015.46