Improved Detection of Ergosterol, Stigmasterol, and Selected Steroids on Silica Coated TLC Plates using Phosphomolybdic Acid Staining

Improved Detection of Ergosterol, Stigmasterol, and Selected Steroids on Silica Coated TLC Plates using Phosphomolybdic Acid Staining

In this work, improved detection of selected steroids on silica coated TLC plates using post-run derivatisation with phosphomolybdic acid (PMA) was investigated. Particularly, spot visualization of ergosterol, stigmasterol, dihydrocholesterol, 4-cholesten-3-one, cholecalciferol, and cholesterol acet...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies Vol. 30; no. 17; pp. 2629 - 2634
Main Authors: Zarzycki, P. K., Baran, M., Włodarczyk, E., Bartoszuk, M. A.
Format: Journal Article
Language:English
Published: Colchester Taylor & Francis Group 01-08-2007
Taylor & Francis
Subjects:
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Densitometry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Other biological molecules
Other chromatographic methods
Phosphomolybdic acid (PMA)
Spots visualization
Steroids
Temperature
Terpenes, steroids. Hormones
Thin-layer chromatography
Steroid
Ergosterol
Temperature
Chemical analysis
Staining
Molybdophosphoric acid
Phosphomolybdic acid (PMA)
Thin layer chromatography
Stigmasterol
Vitamin D
Spots visualization
Thin-layer chromatography
Densitometry
Detection
Colecalciferol
Sterol
Steroids
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Summary:In this work, improved detection of selected steroids on silica coated TLC plates using post-run derivatisation with phosphomolybdic acid (PMA) was investigated. Particularly, spot visualization of ergosterol, stigmasterol, dihydrocholesterol, 4-cholesten-3-one, cholecalciferol, and cholesterol acetate was studied. It has been found that the quantitative effect of PMA dyeing is strongly time and temperature dependent. Contrary to the data reported in literature, our experimental work revealed that the best conditions for robust detection of analytes investigated can be expected if the silica coated chromatographic plates are heated within a relatively low temperature region, between 40 and 80°C and for more than 20 minutes.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826070701540647