A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia

Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying t...

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Bibliographic Details
Published in:	Viruses Vol. 12; no. 4; p. 450
Main Authors:	Bilger, Andrea, King, Renee E, Schroeder, Josh P, Piette, Jared T, Hinshaw, Louis A, Kurth, Andrew D, AlRamahi, Ronnie W, Barthel, Matthew V, Ward-Shaw, Ella T, Buehler, Darya, Masters, Kristyn S, Thibeault, Susan L, Lambert, Paul F
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 16-04-2020 MDPI
Subjects:	Allergies Anesthesia Animal research models Animals Biopsy Cervical cancer Deoxyribonucleic acid Design Disease Models, Animal DNA Endoscopy Epithelium Genomes Head and neck Head and neck cancer House mouse Human papillomavirus Humans Infections Inventors Isoflurane Keratin Laboratory animals Mice mouse Mouth Nasal Mucosa - pathology Nasal Mucosa - virology OPSCC Oropharyngeal Neoplasms - etiology Oropharyngeal Neoplasms - pathology Oropharyngolaryngeal carcinoma Oropharynx Oropharynx - pathology Oropharynx - virology Palate Papillomaviridae - physiology papillomavirus Papillomavirus infections Papillomavirus Infections - complications Papillomavirus Infections - virology Physiological aspects Squamous cell carcinoma Squamous Cell Carcinoma of Head and Neck - etiology Squamous Cell Carcinoma of Head and Neck - pathology Squamous cells throat Tongue Tumorigenesis Tumors Warts United States St Louis Missouri United States > US head-and-neck mouse throat tumor endoscopy papillomavirus OPSCC oropharynx MmuPV1 anesthesia
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Summary:	Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying the development and treatment of these tumors. We have developed an efficient system using the mouse papillomavirus (MmuPV1) to generate dysplastic oropharyngeal lesions, including tumors, in the soft palate and the base of the tongue of two immune-deficient strains of mice. To maximize efficiency and safety during infection and endoscopy, we have designed a nose cone for isoflurane-induced anesthesia that takes advantage of a mouse's need to breathe nasally and has a large window for oral manipulations. To reach and infect the oropharynx efficiently, we have repurposed the Greer Pick allergy testing device as a virus delivery tool. We show that the Pick can be used to infect the epithelium of the soft palate and the base of the tongue of mice directly, without prior scarification. The ability to induce and track oropharyngeal papillomavirus-induced tumors in the mouse, easily and robustly, will facilitate the study of oropharyngeal tumorigenesis and potential treatments.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v12040450