Effects of TGF-beta1, PDGF-BB, and IGF-1 on the rate of proliferation and adhesion of a periodontal ligament cell lineage in vitro

Considering the role of growth factors in periodontal regeneration, the aim of this study was to evaluate the influence of platelet-derived growth factor (PDGF)-BB, insulin-like growth factor (IGF)-1, and transforming growth factor-beta 1 (TGF-beta1), alone or in combination, on the rate of prolifer...

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Published in:	Journal of periodontology (1970) Vol. 78; no. 10; pp. 2007 - 2017
Main Authors:	Sant'Ana, Adriana C P, Marques, Márcia M, Barroso, T Emildre C, Passanezi, Euloir, de Rezende, Maria Lúcia Rubo
Format:	Journal Article
Language:	English
Published:	United States 01-10-2007
Subjects:	Adult Aged Analysis of Variance Cell Adhesion - drug effects Cell Lineage Cell Proliferation - drug effects Cells, Cultured Dental Scaling Dentistry Female Fibroblasts - drug effects Fibroblasts - physiology Humans Insulin-Like Growth Factor I - pharmacology Male Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - physiology Middle Aged Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - physiology Platelet-Derived Growth Factor - pharmacology Proto-Oncogene Proteins c-sis Regeneration - drug effects Statistics, Nonparametric Tooth Root Transforming Growth Factor beta1 - pharmacology
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Summary:	Considering the role of growth factors in periodontal regeneration, the aim of this study was to evaluate the influence of platelet-derived growth factor (PDGF)-BB, insulin-like growth factor (IGF)-1, and transforming growth factor-beta 1 (TGF-beta1), alone or in combination, on the rate of proliferation and adhesion of periodontal ligament (PDL) cells in vitro. After establishment and characterization of a primary culture of PDL cells, 72 culture dishes were plated with 10(3) cells distributed among four test groups and a control group. Test groups had PDGF-BB, TGF-beta1, IGF-1, or a combination of all three added to the culture medium, whereas the control group received no growth factor. The samples were counted in triplicate 1, 3, 5, and 7 days after seeding. For the adhesion assay, 14 patients provided 30 root fragments distributed among 10 groups: scaling and root planing (SRP), SRP + growth factors, SRP + citric acid plus tetracycline (CA+T), and SRP + (CA+T) + growth factors. The data were evaluated statistically by analysis of variance complemented by Tukey, Dunnett, and Student-Newman-Keuels methods. Maximum rates of proliferation were observed at day 3 for all groups. TGF-beta1 induced a 344.17% +/- 58.80% increased proliferation rate over control (P < 0.05), followed by the combination (277.5% +/- 29.38%), PDGF-BB (238.79% +/- 5.79%), and IGF-1 (233.16% +/- 19.19%). Groups treated by (CA+T) showed increased numbers of cells attached to root fragments, especially SRP + (CA+T) + combination (13.25 +/- 1.79), with significant differences (P < 0.05) from groups treated only by SRP. This combination of growth factors stimulated a mitogenic response and favored the adhesion of PDL cells in vitro, suggesting its possible role in periodontal regeneration.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3492