Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls

Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls

Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HL...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo Vol. 65; pp. e14 - 8
Main Authors: Pereira-Manfro, Wânia Ferraz, Silva, Giselle Pereira da, Costa, Priscilla Ramos, Costa, Dayane Alves, Ferreira, Bianca da Silva, Barreto, Daniela Mena, Frota, Ana Cristina Cisne, Hofer, Cristina Barroso, Kallas, Esper Georges, Milagres, Lucimar Gonçalves
Format: Journal Article
Language:English
Published: Brazil Instituto de Medicina Tropical de Sao Paulo 01-01-2023
Instituto de Medicina Tropical
Instituto de Medicina Tropical de São Paulo
Subjects:
Adolescent
Brief Communication
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Child
Drug therapy
Flow cytometry
Genotype & phenotype
HIV
HIV Infections - drug therapy
HLA-DR Antigens - therapeutic use
Human immunodeficiency virus
Humans
Immunoglobulins
Infections
Lymphocytes
Pediatrics
Programmed Cell Death 1 Receptor - therapeutic use
Receptors, Immunologic - therapeutic use
Senescence
Software
Teenagers
TROPICAL MEDICINE
Vaccines
Senescence
Children
HIV-1 infection
CD8 T cells
Immune exhaustion
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Summary:Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HLA-DR), CD38, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), programmed death 1 (PD-1) and CD57 by flow cytometry, during approximately one year. Eleven HIV-infected (HI) and nine HIV-uninfected (HU) children/adolescents who received two doses or one dose of meningococcal C conjugate vaccine (MenC), respectively, were involved in this study. Blood samples were collected before the immunization (T0), 1-2 months after the first dose (T1), and 1-2 months after the second dose (T2), which was administered approximately one year after the first one. HI patients not receiving combined antiretroviral therapy (cART) showed a higher frequency of CD8 T cells TIGIT+, PD-1+ or CD57+, as well as a higher frequency of CD8 T cells co-expressing CD38/HLA-DR/TIGIT or CD38/HLA-DR/PD-1 when compared to HI treated or HU individuals, at all times that they were assessed. CD8 T cells co-expressing CD38/DR/TIGIT were inversely correlated with the CD4/CD8 ratio but positively associated with viral load. The co-expression of CD38/DR/TIGIT or CD38/DR/PD-1 on CD8 T cells was also inversely associated with the CD4 T cells expressing co-stimulatory molecules CD127/CD28. The results showed a higher expression of exhaustion/senescence markers on CD8 T cells of untreated HI children/adolescents and its correlations with viral load.
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The authors declare no conflict of interests.
CONFLICT OF INTERESTS
ISSN:1678-9946
0036-4665
1678-9946
DOI:10.1590/S1678-9946202365014