Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus)
Infection with Helicobacter pylori strains containing high number of EPIYA-C phosphorylation sites in the CagA is associated with significant gastritis and increased risk of developing pre-malignant gastric lesions and gastric carcinoma. However, these findings have not been reproduced in animal mod...
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| Published in: | European journal of histochemistry Vol. 59; no. 2; p. 2489 |
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| Main Authors: | , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Italy
PAGEPress Publications
27-04-2015
PAGEPress Publications, Pavia, Italy |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Infection with Helicobacter pylori strains containing high number of EPIYA-C phosphorylation sites in the CagA is associated with significant gastritis and increased risk of developing pre-malignant gastric lesions and gastric carcinoma. However, these findings have not been reproduced in animal models yet. Therefore, we investigated the effect on the gastric mucosa of Mongolian gerbil (Meriones unguiculatus) infected with CagA-positive H. pylori strains exhibiting one or three EPIYA-C phosphorilation sites. Mongolian gerbils were inoculated with H. pylori clonal isolates containing one or three EPIYA-C phosphorylation sites. Control group was composed by uninfected animals challenged with Brucella broth alone. Gastric fragments were evaluated by the modified Sydney System and digital morphometry. Clonal relatedness between the isolates was considered by the identical RAPD-PCR profiles and sequencing of five housekeeping genes, vacA i/d region and of oipA. The other virulence markers were present in both isolates (vacA s1i1d1m1, iceA2, and intact dupA). CagA of both isolates was translocated and phosphorylated in AGS cells. After 45 days of infection, there was a significant increase in the number of inflammatory cells and in the area of the lamina propria in the infected animals, notably in those infected by the CagA-positive strain with three EPIYA-C phosphorylation sites. After six months of infection, a high number of EPIYA-C phosphorylation sites was associated with progressive increase in the intensity of gastritis and in the area of the lamina propria. Atrophy, intestinal metaplasia, and dysplasia were also observed more frequently in animals infected with the CagA-positive isolate with three EPIYA-C sites. We conclude that infection with H. pylori strain carrying a high number of CagA EPIYA-C phosphorylation sites is associated with more severe gastric lesions in an animal model of H. pylori infection. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Contributions: SAB, ADG, DMMQ, DNA extraction, RUT, analysis of CagA translocation and DNA sequencing; PVTV, IDA, classification and grading of gastritis by the Sydney system and macroscopic analysis of the gastric mucosa; MFJ, MVC, AETD, CMB, RCB, inoculation of the animals, necropsy and histopathological techniques; MFJ, FMSO, AACC, LOP, immunohistochemical techniques; MVC, AACC, MFJ, morphometric analysis of the inflammatory infiltrate and the area of the antral lamina propria; DMMQ, Data analysis; DMMQ, MVC, MFJ, wrote the paper; DMMQ, MVC, IDA,designed the study, supervised the laboratory works. All authors read and approved the manuscript. Conflict of interest: the authors declare no conflict of interest. |
| ISSN: | 1121-760X 2038-8306 |
| DOI: | 10.4081/ejh.2015.2489 |