A Theiler’s virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined by genome detection using real-time RT-PCR and expression of L protein

Cells from various tissues and species are able to bind to Theiler’s virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and show...

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Bibliographic Details
Published in:	Archives of virology Vol. 157; no. 12; pp. 2437 - 2440
Main Authors:	Velloso, Alvaro Jorge, da Silva Junior, Haroldo Cid, Santos, Eneida Almeida, Baroni, Eliane Barbosa, de Moraes, Marcia Terezinha Baroni
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-12-2012 Springer Springer Nature B.V
Subjects:	Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Brief Report Cell Line Cricetinae Cytopathogenic Effect, Viral Encephalomyelitis Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral - physiology Genetic Variation Genome, Viral Genomes Infections Infectious Diseases Medical Microbiology Microbiology Miscellaneous Polyclonal antibodies Proteins Real-Time Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - methods Theiler's encephalomyelitis virus Theilovirus - classification Theilovirus - genetics Viral Proteins - genetics Viral Proteins - metabolism Virology Virus Replication Viruses Brazil United States > US Rio de Janeiro Brazil Subgroup Strain BeAn Virus Persistence Profile 3A3B Region BeAn Strain Virus Cardiovirus Picornaviridae Theiler murine encephalomyelitis virus Detection Genome Real time Reverse transcription polymerase chain reaction Protein Strain
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Summary:	Cells from various tissues and species are able to bind to Theiler’s virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and showed a persistent profile. In order to follow the in vitro infection, real-time RT-PCR to detect the IRES, L* and 3A3B regions of the Theiler’s virus genome was carried out in the first and last passages. In addition, the expression of L* protein was detected. These findings confirm the persistence of the virus in vitro , even in the absence of cytopathic effect (CPE).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-012-1434-4