Growth, biogenic amine production and tyrDC transcription of Enterococcus faecalis in synthetic medium containing defined amino acid concentrations

Aims The tyraminogenic potential of the strains Enterococcus faecalis EF37 and ATCC 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures. Methods and Results Enterococci growth and the production of biogenic amines (BA) were...

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Bibliographic Details
Published in:	Journal of applied microbiology Vol. 122; no. 4; pp. 1078 - 1091
Main Authors:	Bargossi, E., Tabanelli, G., Montanari, C., Gatto, V., Chinnici, F., Gardini, F., Torriani, S.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-04-2017
Subjects:	Amino acids Bacteria Biogenic Amines - biosynthesis Culture Media Enterococcus faecalis Enterococcus faecalis - enzymology Enterococcus faecalis - genetics Enterococcus faecalis - growth & development Enterococcus faecalis - metabolism fermentation biotechnology fermented foods food safety gene expression lactic acid bacteria metabolic processes Metabolism Microbiology Phenylalanine - metabolism Transcription factors Transcription, Genetic Tyramine - biosynthesis Tyrosine - metabolism Tyrosine Decarboxylase - biosynthesis Tyrosine Decarboxylase - genetics food safety fermented foods fermentation biotechnology metabolic processes lactic acid bacteria gene expression
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Summary:	Aims The tyraminogenic potential of the strains Enterococcus faecalis EF37 and ATCC 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures. Methods and Results Enterococci growth and the production of biogenic amines (BA) were evaluated in relation to their pre‐growth in medium containing tyrosine. Significant differences between the two strains were evidenced at metabolic level. Both the pre‐adapted strains grew faster in all the tested conditions, independently of the presence of the precursor. Temperatures of 30 and 40°C positively affected the growth parameters. The tyrosine decarboxylase (tyrDC) activity of the strain EF37 was positively affected by pre‐adaptation, while ATCC 29212 showed a faster and higher tyramine accumulation with not‐adapted cells. The expression analysis of the gene tyrDC confirmed the influence of the growth conditions on gene transcription. Conclusions The small differences found between the two strains in the maximum transcript level reached rapidly after the inoculum and the different behaviour in the tyramine accumulation suggested the possible involvement of complex regulation mechanisms on the tyrDC or on the membrane transport systems, which could affect the different BA accumulation trend. Significance and Impact of the Study This study gives deeper insight into the metabolic regulation of tyrDC activity of enterococci.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1364-5072 1365-2672
DOI:	10.1111/jam.13406