Search Results - "Barfield, Robyn"

Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates by Barfield, Robyn M, Rabuka, David

“…Enzymatic modification of proteins can generate uniquely reactive chemical functionality, enabling site-specific reactions on the protein surface…”
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Tandem-Cleavage Linkers Improve the In Vivo Stability and Tolerability of Antibody–Drug Conjugates by Chuprakov, Stepan, Ogunkoya, Ayodele O, Barfield, Robyn M, Bauzon, Maxine, Hickle, Colin, Kim, Yun Cheol, Yeo, Dominick, Zhang, Fangjiu, Rabuka, David, Drake, Penelope M

“…Although peptide motifs represent the majority of cleavable linkers used in clinical-stage antibody–drug conjugates (ADCs), the sequences are often sensitive…”
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Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes by Drake, Penelope M, Albers, Aaron E, Baker, Jeanne, Banas, Stefanie, Barfield, Robyn M, Bhat, Abhijit S, de Hart, Gregory W, Garofalo, Albert W, Holder, Patrick, Jones, Lesley C, Kudirka, Romas, McFarland, Jesse, Zmolek, Wes, Rabuka, David

“…It is becoming increasingly clear that site-specific conjugation offers significant advantages over conventional conjugation chemistries used to make…”
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Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag by Hudak, Jason E., Barfield, Robyn M., de Hart, Gregory W., Grob, Patricia, Nogales, Eva, Bertozzi, Carolyn R., Rabuka, David

“…Clicking into place: Cu‐free click chemistry is combined with the aldehyde tag protein modification strategy to produce heterobifunctional protein fusions…”
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Site-Specific Bioconjugation Using SMARTag ® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody-Drug Conjugates by Liu, Junjie, Barfield, Robyn M, Rabuka, David

“…As a critical feature of the next generation of antibody-drug conjugates (ADCs), site-specific bioconjugation approaches can help to optimize stability,…”
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Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates by Agarwal, Paresh, Kudirka, Romas, Albers, Aaron E, Barfield, Robyn M, de Hart, Gregory W, Drake, Penelope M, Jones, Lesley C, Rabuka, David

“…Aldehyde- and ketone-functionalized biomolecules have found widespread use in biochemical and biotechnological fields. They are typically conjugated with…”
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CRISPR-Cas9 screens identify regulators of antibody–drug conjugate toxicity by Tsui, C. Kimberly, Barfield, Robyn M., Fischer, Curt R., Morgens, David W., Li, Amy, Smith, Benjamin A. H., Gray, Melissa Anne, Bertozzi, Carolyn R., Rabuka, David, Bassik, Michael C.

“…Antibody–drug conjugates (ADCs) selectively deliver chemotherapeutic agents to target cells and are important cancer therapeutics. However, the mechanisms by…”
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Maytansine-bearing antibody-drug conjugates induce in vitro hallmarks of immunogenic cell death selectively in antigen-positive target cells by Bauzon, Maxine, Drake, Penelope M, Barfield, Robyn M, Cornali, Brandon M, Rupniewski, Igor, Rabuka, David

“…Oncology treatment has been revolutionized by the introduction of immune checkpoint inhibitor drugs, which enable 20-40% of patients to generate anti-tumor…”
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Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion by Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bañas, Stefanie, de Hart, Gregory W., Baker, Jeanne, Rabuka, David

“…To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of…”
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A Novel HER2-targeted Antibody–drug Conjugate Offers the Possibility of Clinical Dosing at Trastuzumab-equivalent Exposure Levels by Barfield, Robyn M., Kim, Yun Cheol, Chuprakov, Stepan, Zhang, Fangjiu, Bauzon, Maxine, Ogunkoya, Ayodele O., Yeo, Dominick, Hickle, Colin, Pegram, Mark D., Rabuka, David, Drake, Penelope M.

“…Abstract Trastuzumab and the related ADC, ado-trastuzumab emtansine (T-DM1), both target HER2-overexpressing cells. Together, these drugs have treatment…”
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Hydrophilic Sequence-Defined Cross-Linkers for Antibody–Drug Conjugates by Walker, Joshua A, Sorkin, Michelle R, Ledesma, Francis, Kabaria, Sneha R, Barfield, Robyn M, Rabuka, David, Alabi, Christopher A

“…Antibody–drug conjugates (ADCs) are an established modality for the tissue-specific delivery of chemotherapeutics. However, due to the hydrophobic nature of…”
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A simple LC/MRM–MS-based method to quantify free linker-payload in antibody-drug conjugate preparations by Zmolek, Wesley, Bañas, Stefanie, Barfield, Robyn M., Rabuka, David, Drake, Penelope M.

“…•A simple MRM-based method to quantify free linker-payload in an ADC is described.•The approach has an LLOQ of 10nM and requires no special laboratory…”
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The exomer coat complex transports Fus1p to the plasma membrane via a novel plasma membrane sorting signal in yeast by Barfield, Robyn M, Fromme, J Christopher, Schekman, Randy

“…Sorting of transmembrane cargo proteins to different cellular compartments is mediated by sorting signals that are recognized by coat proteins involved in…”
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CAT-02-106, a Site-Specifically Conjugated Anti-CD22 Antibody Bearing an MDR1-Resistant Maytansine Payload Yields Excellent Efficacy and Safety in Preclinical Models by Drake, Penelope M, Carlson, Adam, McFarland, Jesse M, Bañas, Stefanie, Barfield, Robyn M, Zmolek, Wesley, Kim, Yun Cheol, Huang, Betty C B, Kudirka, Romas, Rabuka, David

“…Hematologically derived tumors make up ∼10% of all newly diagnosed cancer cases in the United States. Of these, the non-Hodgkin lymphoma (NHL) designation…”
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Exploring the effects of linker composition on site-specifically modified antibody–drug conjugates by Albers, Aaron E., Garofalo, Albert W., Drake, Penelope M., Kudirka, Romas, de Hart, Gregory W., Barfield, Robyn M., Baker, Jeanne, Banas, Stefanie, Rabuka, David

“…In the context of antibody–drug conjugates (ADCs), noncleavable linkers provide a means to deliver cytotoxic small molecules to cell targets while reducing…”
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Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II) by York, Dona, Baker, Jeanne, Holder, Patrick G, Jones, Lesley C, Drake, Penelope M, Barfield, Robyn M, Bleck, Gregory T, Rabuka, David

“…The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other…”
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Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions by Huang, Betty C B, Kim, Yun Cheol, Bañas, Stefanie, Barfield, Robyn M, Drake, Penelope M, Rupniewski, Igor, Haskins, William E, Rabuka, David

“…The advantages of site-specific over stochastic bioconjugation technologies include homogeneity of product, minimal perturbation of protein structure/function,…”
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Site-Specific Tandem Knoevenagel Condensation–Michael Addition To Generate Antibody–Drug Conjugates by Kudirka, Romas A, Barfield, Robyn M, McFarland, Jesse M, Drake, Penelope M, Carlson, Adam, Bañas, Stefanie, Zmolek, Wes, Garofalo, Albert W, Rabuka, David

“…Expanded ligation techniques are sorely needed to generate unique linkages for the growing field of functionally enhanced proteins. To address this need, we…”
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Phosphorylation of Chs2p regulates interaction with COPII by Jakobsen, Mia Kyed, Cheng, Zhiliang, Lam, Sheung Kwan, Roth-Johnson, Elizabeth, Barfield, Robyn M, Schekman, Randy

“…Trafficking of the chitin synthase Chs2p from the endoplasmic reticulum (ER) to the bud-neck in late mitosis is tightly regulated by the cell cycle via…”
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Antibody-Drug Conjugates Targeting the Urokinase Receptor (uPAR) as a Possible Treatment of Aggressive Breast Cancer by Harel, Efrat T, Drake, Penelope M, Barfield, Robyn M, Lui, Irene, Farr-Jones, Shauna, Van't Veer, Laura, Gartner, Zev J, Green, Evan M, Lourenço, André Luiz, Cheng, Yifan, Hann, Byron C, Rabuka, David, Craik, Charles S

“…A promising molecular target for aggressive cancers is the urokinase receptor (uPAR). A fully human, recombinant antibody that binds uPAR to form a stable…”
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