Search Results - "Barfield, M."

Synthesis of Heterobifunctional Protein Fusions Using Copper-Free Click Chemistry and the Aldehyde Tag by Hudak, Jason E., Barfield, Robyn M., de Hart, Gregory W., Grob, Patricia, Nogales, Eva, Bertozzi, Carolyn R., Rabuka, David

“…Clicking into place: Cu‐free click chemistry is combined with the aldehyde tag protein modification strategy to produce heterobifunctional protein fusions…”
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Maytansine-bearing antibody-drug conjugates induce in vitro hallmarks of immunogenic cell death selectively in antigen-positive target cells by Bauzon, Maxine, Drake, Penelope M, Barfield, Robyn M, Cornali, Brandon M, Rupniewski, Igor, Rabuka, David

“…Oncology treatment has been revolutionized by the introduction of immune checkpoint inhibitor drugs, which enable 20-40% of patients to generate anti-tumor…”
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Current Approaches for Absorption, Distribution, Metabolism, and Excretion Characterization of Antibody-Drug Conjugates: An Industry White Paper by Kraynov, Eugenia, Kamath, Amrita V, Walles, Markus, Tarcsa, Edit, Deslandes, Antoine, Iyer, Ramaswamy A, Datta-Mannan, Amita, Sriraman, Priya, Bairlein, Michaela, Yang, Johnny J, Barfield, Matthew, Xiao, Guangqing, Escandon, Enrique, Wang, Weirong, Rock, Dan A, Chemuturi, Nagendra V, Moore, David J

“…An antibody-drug conjugate (ADC) is a unique therapeutic modality composed of a highly potent drug molecule conjugated to a monoclonal antibody. As the number…”
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The phenomenology of niche evolution via quantitative traits in a ‘black-hole’ sink by Holt, R. D., Gomulkiewicz, R., Barfield, M.

“…Previous studies of adaptive evolution in sink habitats (in which isolated populations of a species cannot persist deterministically) have highlighted the…”
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Antibody-Drug Conjugates Targeting the Urokinase Receptor (uPAR) as a Possible Treatment of Aggressive Breast Cancer by Harel, Efrat T, Drake, Penelope M, Barfield, Robyn M, Lui, Irene, Farr-Jones, Shauna, Van't Veer, Laura, Gartner, Zev J, Green, Evan M, Lourenço, André Luiz, Cheng, Yifan, Hann, Byron C, Rabuka, David, Craik, Charles S

“…A promising molecular target for aggressive cancers is the urokinase receptor (uPAR). A fully human, recombinant antibody that binds uPAR to form a stable…”
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Use of DBS sample collection to determine circulating drug concentrations in clinical trials: practicalities and considerations by Spooner, Neil, Ramakrishnan, Y, Barfield, M, Dewit, O, Miller, S

“…A clinical investigation was performed into the practicalities of the collection of blood samples for the determination of drug exposures on filter paper,…”
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Effects of Compost on the Chemistry of an Urban Upper Piedmont South Carolina Soil by Mikhailova, E. A., Schlautman, M. A., Darnault, C. J. G., Sharp, J. L., Post, C. J., Hall, K. C., Ouzts, E. V., Barfield, M. A.

“…Composting is an effective method to improve the fertility of degraded soils. However, the potential beneficial effects of composting on the fertility of urban…”
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Systematic LC/MS/MS Investigations for the IND-Enabling Extended Characterization of Antibody–Drug Conjugate Modifications by Linz, Thomas, Yeo, Dominick, Hong, Qiuting, Zmolek, Wesley, McFarland, Jesse, Barfield, Robyn, Haskins, William, Rabuka, David

“…We hypothesized that systematic liquid chromatography-tandem mass spectrometry investigations of an antibody–drug conjugate (ADC), its small and large…”
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A DFT Study of the Interresidue Dependencies of Scalar J-Coupling and Magnetic Shielding in the Hydrogen-Bonding Regions of a DNA Triplex by Barfield, Michael, Dingley, Andrew J, Feigon, Juli, Grzesiek, Stephan

“…Scalar coupling constants and magnetic shieldings in the imino hydrogen-bonding region of Hoogsteen−Watson−Crick T·A-T and C+·G−C triplets have been calculated…”
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Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates by Barfield, Robyn M, Rabuka, David

“…Enzymatic modification of proteins can generate uniquely reactive chemical functionality, enabling site-specific reactions on the protein surface…”
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Tandem-Cleavage Linkers Improve the In Vivo Stability and Tolerability of Antibody–Drug Conjugates by Chuprakov, Stepan, Ogunkoya, Ayodele O, Barfield, Robyn M, Bauzon, Maxine, Hickle, Colin, Kim, Yun Cheol, Yeo, Dominick, Zhang, Fangjiu, Rabuka, David, Drake, Penelope M

“…Although peptide motifs represent the majority of cleavable linkers used in clinical-stage antibody–drug conjugates (ADCs), the sequences are often sensitive…”
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Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes by Drake, Penelope M, Albers, Aaron E, Baker, Jeanne, Banas, Stefanie, Barfield, Robyn M, Bhat, Abhijit S, de Hart, Gregory W, Garofalo, Albert W, Holder, Patrick, Jones, Lesley C, Kudirka, Romas, McFarland, Jesse, Zmolek, Wes, Rabuka, David

“…It is becoming increasingly clear that site-specific conjugation offers significant advantages over conventional conjugation chemistries used to make…”
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Site-Specific Bioconjugation Using SMARTag ® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody-Drug Conjugates by Liu, Junjie, Barfield, Robyn M, Rabuka, David

“…As a critical feature of the next generation of antibody-drug conjugates (ADCs), site-specific bioconjugation approaches can help to optimize stability,…”
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Multiple acquired portosystemic shunts in a cat secondary to chronic diaphragmatic rupture by Barfield, Dominic M, Gibson, Andrew D, Lipscomb, Vicky J

“…A cat with a chronic diaphragmatic rupture presented with neurological signs, including twitching and focal seizures. Blood ammonia level was markedly elevated…”
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Hydrazino-Pictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates by Agarwal, Paresh, Kudirka, Romas, Albers, Aaron E, Barfield, Robyn M, de Hart, Gregory W, Drake, Penelope M, Jones, Lesley C, Rabuka, David

“…Aldehyde- and ketone-functionalized biomolecules have found widespread use in biochemical and biotechnological fields. They are typically conjugated with…”
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CRISPR-Cas9 screens identify regulators of antibody–drug conjugate toxicity by Tsui, C. Kimberly, Barfield, Robyn M., Fischer, Curt R., Morgens, David W., Li, Amy, Smith, Benjamin A. H., Gray, Melissa Anne, Bertozzi, Carolyn R., Rabuka, David, Bassik, Michael C.

“…Antibody–drug conjugates (ADCs) selectively deliver chemotherapeutic agents to target cells and are important cancer therapeutics. However, the mechanisms by…”
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Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion by Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bañas, Stefanie, de Hart, Gregory W., Baker, Jeanne, Rabuka, David

“…To further our aim of synthesizing aldehyde-tagged proteins for research and biotechnology applications, we developed methods for recombinant production of…”
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A Novel HER2-targeted Antibody–drug Conjugate Offers the Possibility of Clinical Dosing at Trastuzumab-equivalent Exposure Levels by Barfield, Robyn M., Kim, Yun Cheol, Chuprakov, Stepan, Zhang, Fangjiu, Bauzon, Maxine, Ogunkoya, Ayodele O., Yeo, Dominick, Hickle, Colin, Pegram, Mark D., Rabuka, David, Drake, Penelope M.

“…Abstract Trastuzumab and the related ADC, ado-trastuzumab emtansine (T-DM1), both target HER2-overexpressing cells. Together, these drugs have treatment…”
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Hydrophilic Sequence-Defined Cross-Linkers for Antibody–Drug Conjugates by Walker, Joshua A, Sorkin, Michelle R, Ledesma, Francis, Kabaria, Sneha R, Barfield, Robyn M, Rabuka, David, Alabi, Christopher A

“…Antibody–drug conjugates (ADCs) are an established modality for the tissue-specific delivery of chemotherapeutics. However, due to the hydrophobic nature of…”
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A simple LC/MRM–MS-based method to quantify free linker-payload in antibody-drug conjugate preparations by Zmolek, Wesley, Bañas, Stefanie, Barfield, Robyn M., Rabuka, David, Drake, Penelope M.

“…•A simple MRM-based method to quantify free linker-payload in an ADC is described.•The approach has an LLOQ of 10nM and requires no special laboratory…”
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