Topical anti-inflammatory activity of Eugenia brasiliensis Lam. (Myrtaceae) leaves

Eugenia brasiliensis Lam., a plant from the south of Brazil, is used in the popular medicine for rheumatism treatment. This study reports that topical application of hydroalcoholic extract, fractions and isolated compounds from E. brasiliensis caused an inhibition of ear oedema in response to topica...

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Bibliographic Details
Published in:	Journal of pharmacy and pharmacology Vol. 60; no. 4; pp. 479 - 487
Main Authors:	Pietrovski, Evelise Fernandes, Magina, Michele Debiasi Alberton, Gomig, Franciane, Pietrovski, Caroline Fernandes, Micke, Gustavo Amadeu, Barcellos, Michele, Pizzolatti, Moacir Geraldo, Cabrini, Daniela Almeida, Brighente, Inês Maria Costa, Otuki, Michel Fleith
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-04-2008
Subjects:	Administration, Topical Animals Anti-Inflammatory Agents, Non-Steroidal - chemistry Anti-Inflammatory Agents, Non-Steroidal - isolation & purification Anti-Inflammatory Agents, Non-Steroidal - therapeutic use Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - therapeutic use Arachidonic Acid Brazil Catechin - analogs & derivatives Catechin - chemistry Catechin - isolation & purification Catechin - therapeutic use Croton Oil Dermatitis - drug therapy Dermatitis - etiology Dose-Response Relationship, Drug Edema - chemically induced Edema - drug therapy Electrophoresis, Capillary Male Mice Phytotherapy Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - therapeutic use Plant Leaves - chemistry Quercetin - chemistry Quercetin - isolation & purification Quercetin - therapeutic use Syzygium - chemistry
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Summary:	Eugenia brasiliensis Lam., a plant from the south of Brazil, is used in the popular medicine for rheumatism treatment. This study reports that topical application of hydroalcoholic extract, fractions and isolated compounds from E. brasiliensis caused an inhibition of ear oedema in response to topical application of croton oil on the mouse ear. For oedema inhibition, the estimated ID50 values (dose reducing the inflammatory response by 50% relative to the control value) for hydroalcoholic extract and fractions (hexane, ethyl acetate and dichloromethane) were 0.17, 0.29, 0.13 and 0.14 mg/ear, respectively, with inhibition of 79 ± 7%, 87 ± 6%, 88 ± 5% and 96 ± 2%, respectively. Isolated phenolic compounds (quercetin, catechin and gallocatechin) were also effective in inhibiting the oedema (inhibition of 61 ± 5%, 66 ± 2% and 37 ± 9%, respectively). Moreover, both extract and isolated compounds caused inhibition of polymorphonuclear cells influx (inhibition of 85 ± 6%, 81 ± 5%, 73 ± 6% and 76 ± 6%, respectively). The histological analysis of the ear tissue clearly confirmed that the extract and compounds of E. brasiliensis inhibited the influx of polymorphonuclear cells to mouse ear skin after application of croton oil. Furthermore, hydroalcoholic extract was also effective in inhibiting the arachidonic acid‐mediated mouse ear oedema (ID50 value was 1.94 mg/ear and inhibition of 60 ± 7%). Therefore, these results consistently support the notion that E. brasiliensis possesses topical anti‐inflammatory activity.
Bibliography:	ark:/67375/WNG-Q72MLLB8-8 ArticleID:JPHP1759 istex:9488837AFA644C37603667E0DCD7D30EE694A58A Michel F. Otuki receives a post‐doctoral fellowship from UFPR/CAPES/ PRODOC. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-3573 2042-7158
DOI:	10.1211/jpp.60.4.0011