Protective effect of Chresta martii extract on ethanol-induced gastropathy depends on alpha-2 adrenoceptors pathways but not on nitric oxide, prostaglandins or opioids

Species of Chresta genus- are recognized by the population of northeastern Brazil as traditional herbs used to treat gastric diseases and other disorders. This work aimed to find out the action mechanism of Chresta martii hydro alcoholic extract gastro protective effect in the model of ethanol-induc...

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Bibliographic Details
Published in:	Journal of ethnopharmacology Vol. 142; no. 1; pp. 206 - 212
Main Authors:	Rodrigues e Silva, Antonio Alfredo, Marques Bezerra, Mirna, Vasconcelos Chaves, Hellíada, de Paulo Teixeira Pinto, Vicente, de Souza Franco, Eryvelton, Magalhães Vieira, Ângela, Barbosa Araújo, Edeline, Cunha Rios, Lívia, Resende Leite, Ana Catarina, de Sousa Maia, Maria Bernadete
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 26-06-2012
Subjects:	Adrenergic alpha-2 Receptor Agonists - pharmacology Alpha-2 adrenoceptors Analgesics, Opioid Animals Asteraceae Chresta Clonidine - pharmacology Ethanol Flowers Gastropathy Hemoglobins - metabolism Male Mice Nitric Oxide Plant Extracts - therapeutic use Plant Leaves Prostaglandins Protective Agents - therapeutic use Receptors, Adrenergic, alpha-2 - metabolism Stomach Diseases - chemically induced Stomach Diseases - drug therapy Stomach Diseases - metabolism Stomach Diseases - pathology Sulfhydryl Compounds - metabolism Gastropathy Alpha-2 adrenoceptors Chresta
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Summary:	Species of Chresta genus- are recognized by the population of northeastern Brazil as traditional herbs used to treat gastric diseases and other disorders. This work aimed to find out the action mechanism of Chresta martii hydro alcoholic extract gastro protective effect in the model of ethanol-induced gastropathy. Gastropathy was assessed by percentual damaged area determination in photographs of mice opened stomachs. Fasted mice treated with ethanol 99.9% (0.2 ml/animal, p.o.) were pre-treated with Chresta martii hydro alcoholic extract (HAE) (50, 100 or 200 mg/kg, p.o.), ranitidine (80 mg/kg, p.o.) or saline (5 ml/kg; p.o.) in different experimental sets, in which pharmacological tools (naloxone, indomethacin, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) or yohimbine) were added in order to clarify a possible action mechanism. Animals were sacrificed 30 min after ethanol challenge to stomach analysis. Determination of non-protein sulfhydryl groups and tissue hemoglobin, besides histological assessment (H&E) were taken to fully characterize the HAE gastro protective effect. HAE (100 and 200 mg/kg) was able to protect mucosa against ethanol gastropathy in presence of three (naloxone, indomethacin and L-NAME) of four antagonist/inhibitor tools. The HAE effect was reversed only by yohimbine, showing the alpha-2 adrenoceptors participation on gastro protective effect of this extract. HAE histological characteristics, NP-SH and Hb were compatible with the protective effects. HAE possesses gastroprotective effects in an ethanol-induced gastropathy model in mice, corroborating the traditional use of this family of plants to treat gastric disorders. This activity is mediated by alpha-2 adrenoceptors activation, but not by nitric oxide release, opioid receptor activation or prostaglandin synthesis. HAE also has antioxidant activity that is thought to either play a role in this biological activity or to be a byproduct of alpha-2 adrenergic complex activation. [Display omitted]
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-8741 1872-7573
DOI:	10.1016/j.jep.2012.04.042