New sources of resistance to Meloidogyne incognita race 3 in wild cotton accessions and histological characterization of the defence mechanisms

New sources of resistance to Meloidogyne incognita race 3 in wild cotton accessions and histological characterization of the defence mechanisms

Accessions of Gossypium spp., some of them never previously tested, were evaluated for resistance to a local isolate of Meloidogyne incognita race 3 in greenhouse experiments. Nematode infection was characterized based on the galling and egg mass indexes and the reproduction factors (RF). Root‐knot...

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Bibliographic Details
Published in:Plant pathology Vol. 62; no. 5; pp. 1173 - 1183
Main Authors: Mota, F. C., Alves, G. C. S., Giband, M., Gomes, A. C. M. M., Sousa, F. R., Mattos, V. S., Barbosa, V. H. S., Barroso, P. A. V., Nicole, M., Peixoto, J. R., Rocha, M. R., Carneiro, R. M. D. G.
Format: Journal Article
Language:English
Published: Oxford Blackwell 01-10-2013
Wiley Subscription Services, Inc
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Control
Fundamental and applied biological sciences. Psychology
Gossypium
Gossypium spp
host plant resistance
hypersensitive response
Meloidogyne incognita
Nematoda
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Malvaceae
Plant pathology
Gossypium
Fiber crop
Source
Characterization
hypersensitive response
host plant resistance
Pest
Meloidogyne incognita
Dicotyledones
Host plant
Angiospermae
Meloidogynidae
Wild plant
Nematoda
Vegetals
Hypersensitivity
Histology
Gossypium spp
Response
Resistance
Helmintha
Defense mechanism
Nemathelminthia
Spermatophyta
Invertebrata
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Summary:Accessions of Gossypium spp., some of them never previously tested, were evaluated for resistance to a local isolate of Meloidogyne incognita race 3 in greenhouse experiments. Nematode infection was characterized based on the galling and egg mass indexes and the reproduction factors (RF). Root‐knot nematode reproduction among the newly tested accessions varied from highly susceptible (AS0188, AS0189) to moderately resistant (MT123 no. 3), and some accessions showed highly reduced nematode reproduction (CIR1343, CIR1348, Fai Mui). Histological observations of two resistant accessions (G. barbadense CIR1348 and G. hirsutum TX‐25, respectively) showed that resistance occurs through a two‐stage mechanism in the first accession and through a single‐stage mechanism in the second. Parasitism is blocked early after second‐stage juvenile (J2) penetration or during its initial tissue migration (CIR1348) and the development of later‐stage juveniles into female adults is suppressed at a later stage (TX‐25 and CIR1348). Fluorescence and bright light microscopy showed that root cells surrounding nematodes exhibit a hypersensitivity‐like reaction, with the accumulation of presumably phenolic compounds and the presence of necrotic cells that limit the development of nematodes and the formation of giant cells. Underdeveloped giant cells with degenerated cytoplasmic content were found in small numbers in CIR1348 and in large numbers in TX‐25, along with deformed nematodes. The full characterization of the defence mechanisms of novel sources of resistance to the root‐knot nematode in cotton constitutes a first step towards their use in crop improvement.
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ISSN:0032-0862
1365-3059
DOI:10.1111/ppa.12022