Analysis of microarchitectural changes in a mouse temporomandibular joint osteoarthritis model

Abstract Objective Little is known about the natural progression of the disease process of temporomandibular joint (TMJ) osteoarthritis (OA), which affects approximately 1% of the US population. The goal of this study was to examine the early microarchitectural and molecular changes in the condylar...

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Published in:	Archives of oral biology Vol. 54; no. 12; pp. 1091 - 1098
Main Authors:	Chen, J, Gupta, T, Barasz, J.A, Kalajzic, Z, Yeh, W.-C, Drissi, H, Hand, A.R, Wadhwa, S
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-12-2009
Subjects:	Advanced Basic Science Aggrecans - biosynthesis Aggrecans - genetics Animals Biglycan Cartilage, Articular - pathology Collagen Type X - biosynthesis Collagen Type X - genetics Dentistry Disease Models, Animal Extracellular Matrix Proteins - deficiency Fibromodulin Gene Expression Mandibular Condyle - metabolism Mandibular Condyle - pathology Mice Mice, Knockout Microscopy, Electron, Transmission Osteoarthritis Osteoarthritis - metabolism Osteoarthritis - pathology Parathyroid Hormone-Related Protein - biosynthesis Parathyroid Hormone-Related Protein - genetics Proteoglycans - deficiency Subchondral bone Temporomandibular joint Temporomandibular Joint Disorders - metabolism Temporomandibular Joint Disorders - pathology X-Ray Microtomography TMJ OA fibromodulin temporomandibular joint Subchondral bone osteoarthritis BGN biglycan FMOD Fibromodulin Temporomandibular joint Biglycan Osteoarthritis
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Summary:	Abstract Objective Little is known about the natural progression of the disease process of temporomandibular joint (TMJ) osteoarthritis (OA), which affects approximately 1% of the US population. The goal of this study was to examine the early microarchitectural and molecular changes in the condylar cartilage and subchondral bone in biglycan / fibromodulin ( Bgn / Fmod ) double-deficient mice, which develop TMJ-OA at 6 months. Methods TMJs from 3-month-old ( n = 44) and 9-month-old ( n = 52) wild-type (WT n = 46) and Bgn / Fmod ( n = 50) double-deficient mice were evaluated. Micro-CT analysis of the subchondral bone ( n = 24), transmission electron microscopy for condylar cartilage fibril diameters ( n = 26), and real-time PCR analysis for gene expression for bone and cartilage maturation markers ( n = 45) was performed. Results A statistically significant increase in collagen fibril diameter of the condylar cartilage and a decrease in expression of Parathyroid related protein in the mandibular condylar head were observed in the 3-month Bgn / Fmod double-deficient mice compared to WT controls. The 9-month Bgn / Fmod double-deficient mouse demonstrated an increase in bone volume and total volume in subchondral bone, and an increase in the expression of Collagen Type X and Aggrecan in the mandibular condylar head compared to the WT controls. Conclusion We found that changes in the microarchitecture of the condylar cartilage preceded changes in the subchondral bone during OA in the TMJ in Bgn / Fmod double-deficient mice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Co First authors
ISSN:	0003-9969 1879-1506
DOI:	10.1016/j.archoralbio.2009.10.001