Isolation and characterization of vibriophage vB_Vc_SrVc9: an effective agent in preventing Vibrio campbellii infections in brine shrimp nauplii (Artemia franciscana)

Isolation and characterization of vibriophage vB_Vc_SrVc9: an effective agent in preventing Vibrio campbellii infections in brine shrimp nauplii (Artemia franciscana)

Aims This study describes the physicochemical and genomic characterization of phage vB_Vc_SrVc9 and its potential for phage therapy application against a pathogenic Vibrio campbellii strain. Methods and Results A lytic phage vB_Vc_SrVc9 against V. campbellii was isolated from shrimp farm sediment, a...

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Published in:Journal of applied microbiology Vol. 131; no. 1; pp. 36 - 49
Main Authors: Lomelí‐Ortega, C.O., Martínez‐Sández, A.J., Barajas‐Sandoval, D.R., Reyes, A.G., Magallón‐Barajas, F., Veyrand‐Quíros, B., Gannon, L., Harrison, C., Michniewski, S., Millard, A., Quiroz‐Guzmán, E.
Format: Journal Article
Language:English
Published: Cambridge Oxford University Press 01-07-2021
Subjects:
Aquaculture
Artemia
Artemia franciscana
Bacteria
bacteriophage
Brines
Burst size
Environmental conditions
Genomic analysis
Infections
Latent period
lytic
Microbiota
pathogen
phage therapy
Phages
Therapeutic applications
Vibrio
Vibrio campbellii
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Summary:Aims This study describes the physicochemical and genomic characterization of phage vB_Vc_SrVc9 and its potential for phage therapy application against a pathogenic Vibrio campbellii strain. Methods and Results A lytic phage vB_Vc_SrVc9 against V. campbellii was isolated from shrimp farm sediment, and characterized physicochemical and genomically. The use of vB_Vc_SrVc9 phage increased the survival in brine shrimp Artemia franciscana and reduced presumptive V. campbellii to nondetectable numbers. Genomic analysis showed a genome with a single contig of 43·15 kb, with 49 predicted genes and no tRNAs, capable of recognizing and generating complete inhibition zones of three Vibrio sp. Conclusions To our knowledge vB_Vc_SrVc9 is a lytic phage that could be used against Vibrio infections, reducing vibrio presence without any apparent impact over the natural microbiota at the family level in 28 libraries tested. Significance and Impact of the Study vB_Vc_SrVC9 is a novel phage and ecofriendly alternative for therapeutic applications and biotechnological purposes because is stable at different environmental conditions, has the potential to eliminate several strains, and has a short latent period with a good burst size. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria.
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ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14937