Indoor dust acts as an adjuvant to promote sensitization to peanut through the airway
Background There is growing evidence that environmental peanut exposure through non‐oral routes, including the skin and respiratory tract, can result in peanut sensitization. Environmental adjuvants in indoor dust can promote sensitization to inhaled antigens, but whether they contribute to peanut a...
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| Published in: | Clinical and experimental allergy Vol. 49; no. 11; pp. 1500 - 1511 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Wiley Subscription Services, Inc
01-11-2019
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Background
There is growing evidence that environmental peanut exposure through non‐oral routes, including the skin and respiratory tract, can result in peanut sensitization. Environmental adjuvants in indoor dust can promote sensitization to inhaled antigens, but whether they contribute to peanut allergy development is unclear.
Objective
We investigated whether indoor dust promotes airway sensitization to peanut and peanut allergy development in mice.
Methods
Female and male C57BL/6J mice were exposed via the airways to peanut, indoor dust extract, or both for 2 weeks. Mice were then challenged with peanut and assessed for anaphylaxis. Peanut‐specific immunoglobulins, peanut uptake by lung conventional dendritic cells (cDCs), lung innate cytokines, and T cell differentiation in lung‐draining lymph nodes were quantified. Innate cytokine production by primary human bronchial epithelial cells exposed to indoor dust was also determined.
Results
Inhalational exposure to low levels of peanut in combination with indoor dust, but neither alone, resulted in production of peanut‐specific IgE and development of anaphylaxis upon peanut challenge. Indoor dust triggered production of innate cytokines in murine lungs and in primary human bronchial epithelial cells. Additionally, inhaled indoor dust stimulated maturation and migration of peanut‐laden lung type 1 cDCs to draining lymph nodes. Inhalational exposure to peanut and indoor dust induced peanut‐specific T helper 2 cell differentiation and accumulation of T follicular helper cells in draining lymph nodes, which were associated with increased B cell numbers and peanut‐specific immunoglobulin production.
Conclusions & clinical relevance
Indoor dust promotes airway sensitization to peanut and development of peanut allergy in mice. Our findings suggest that environmental adjuvants in indoor dust may be determinants of peanut allergy development in children. |
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| Bibliography: | Funding information The data that support the findings of this study are available from the corresponding author upon reasonable request. Data Availability Statement JMS is funded by a T32 Allergy/Immunology Training Grant (AI007062) through Duke University and University of North Carolina (UNC) at Chapel Hill. This work is supported in part by the American Research Foundation for Nut Allergies (MDK. and JMS), the American Academy of Allergy, Asthma and Immunology Foundation (TPM), the UNC Center for Environmental Health and Susceptibility (TPM and MDK.), the Cystic Fibrosis Foundation (BOUCHE15R0 to SHR), and the NIH (DK065988 to SHR). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
| ISSN: | 0954-7894 1365-2222 |
| DOI: | 10.1111/cea.13486 |