Search Results - "Balo, Aidin R"
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Accessible virtual reality of biomolecular structural models using the Autodesk Molecule Viewer
Published in Nature methods (01-12-2017)Get full text
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Experimental guidelines for trapping paramagnetic reaction intermediates in radical S-adenosylmethionine enzymes
Published in Methods in enzymology (2022)“…Due to their biological importance and functional diversity, radical S-adenosylmethionine (rSAM) enzymes have become popular targets for electron paramagnetic…”
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Trapping a cross-linked lysine–tryptophan radical in the catalytic cycle of the radical SAM enzyme SuiB
Published in Proceedings of the National Academy of Sciences - PNAS (25-05-2021)“…The radical S-adenosylmethionine (rSAM) enzyme SuiB catalyzes the formation of an unusual carbon–carbon bond between the sidechains of lysine (Lys) and…”
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Characterizing SPASM/twitch Domain-Containing Radical SAM Enzymes by EPR Spectroscopy
Published in Applied magnetic resonance (01-05-2022)“…Owing to their importance, diversity and abundance of generated paramagnetic species, radical S -adenosylmethionine (rSAM) enzymes have become popular targets…”
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Electron paramagnetic resonance spectroscopy on G-protein-coupled receptors: Adopting strategies from related model systems
Published in Current opinion in structural biology (01-08-2021)“…Membrane proteins, including ion channels, transporters and G-protein-coupled receptors (GPCRs), play a significant role in various physiological processes…”
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Stationary Phase EPR Spectroscopy for Monitoring Membrane Protein Refolding by Conformational Response
Published in Analytical chemistry (Washington) (02-01-2019)“…Protein production remains a major bottleneck in membrane protein structural biology. In many cases, large-scale recombinant protein expression is either…”
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Toward Precise Interpretation of DEER-Based Distance Distributions: Insights from Structural Characterization of V1 Spin-Labeled Side Chains
Published in Biochemistry (Easton) (20-09-2016)“…Pulsed electron paramagnetic resonance experiments can measure individual distances between two spin-labeled side chains in proteins in the range of ∼1.5–8 nm…”
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A Versatile System for High-Throughput In Situ X‑ray Screening and Data Collection of Soluble and Membrane-Protein Crystals
Published in Crystal growth & design (02-11-2016)“…In recent years, in situ data collection has been a major focus of progress in protein crystallography. Here, we introduce the Mylar in situ method using…”
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Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI(-) cells using a PiggyBac inducible system
Published in Methods in enzymology (2015)“…Rhodopsin is a class A G protein-coupled receptor (GPCR) that provides important insights into the structure and function of the GPCR superfamily. Bovine…”
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Prediction and Selection of Spin Label Rotamers for High-Resolution Insight into Protein Structures by DEER Spectroscopy
Published in Biophysical journal (16-02-2016)Get full text
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Interdomain Conformational Changes in Visual Arrestin upon Binding to Different Forms of Rhodopsin
Published in Biophysical journal (03-02-2017)Get full text
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Exploring Complexes of Rhodopsin and Binding Partners in Virtual Reality
Published in Biophysical journal (03-02-2017)Get full text
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