Non-selective inhibition of mammalian protein kinases by flavinoids in vitro

In vitro, the flavinoids quercetin, morin, and rutin produced comparable inhibition of protein kinase C prepared from bovine brain, the epidermal growth factor (EGF)-stimulated tyrosine specific protein kinase associated with A431 cell membranes, and the cAMP-stimulated protein kinase activity assoc...

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Bibliographic Details
Published in:	Research communications in chemical pathology and pharmacology Vol. 56; no. 1; p. 75
Main Authors:	End, D W, Look, R A, Shaffer, N L, Balles, E A, Persico, F J
Format:	Journal Article
Language:	English
Published:	United States 01-04-1987
Subjects:	Adenosine Triphosphate - metabolism Animals Cell Line Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor - pharmacology Flavonoids - pharmacology Phosphoproteins - metabolism Phosphorylation Protein Kinase C - antagonists & inhibitors Protein Kinase Inhibitors
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Summary:	In vitro, the flavinoids quercetin, morin, and rutin produced comparable inhibition of protein kinase C prepared from bovine brain, the epidermal growth factor (EGF)-stimulated tyrosine specific protein kinase associated with A431 cell membranes, and the cAMP-stimulated protein kinase activity associated with rat brain tubulin. Concentrations producing 50% inhibition (IC50's) were in the 10 microM range. These findings demonstrate the lack of specificity of the flavinoids toward cyclic nucleotide-dependent vs cyclic nucleotide-independent protein kinases.
ISSN:	0034-5164