Anti-reflection cover to control acoustic intensity in in vitro low-intensity ultrasound stimulation of cells

Low-intensity ultrasound stimulation is a technique used in therapeutic ultrasound for bone regeneration. However, the underlying mechanisms are still poorly understood. In vitro studies on cell cultures are implemented to understand the processes involved. To analyze the effects of ultrasonic waves...

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Bibliographic Details
Published in:	Acta acustica Vol. 6; no. 11; p. 11
Main Authors:	Majnooni, Meysam, Doveri, Elise, Baldisser, Jeanne, Long, Vincent, Houles, Julien, Scimeca, Jean-Claude, Momier, David, Guivier-Curien, Carine, Lasaygues, Philippe, Baron, Cécile
Format:	Journal Article
Language:	English
Published:	EDP Sciences 2022
Subjects:	acoustic intensity Acoustics anti-reflection cover cell-free culture medium Mechanics Physics ultrasound stimulation Ultrasound stimulation Anti-reflection cover Cell-free culture medium Acoustic intensity
Online Access:	Get full text
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Summary:	Low-intensity ultrasound stimulation is a technique used in therapeutic ultrasound for bone regeneration. However, the underlying mechanisms are still poorly understood. In vitro studies on cell cultures are implemented to understand the processes involved. To analyze the effects of ultrasonic waves on cells, the control of the delivered acoustic intensity is essential. However, depending on the insonification protocol chosen, multiple reflections and standing waves that form inside the culture medium strongly hinder the estimates. In this work, we propose the development and the experimental validation of an anti-reflection cover. We demonstrate that this custom-designed device is effective in avoiding multiple reflections and makes it possible to artificially replace the layer of culture medium with a large amount of water. Finally, an analytical study of the acoustic intensity delivered to the cells is proposed.
ISSN:	2681-4617 2681-4617
DOI:	10.1051/aacus/2022007