Simultaneous liquid chromatographic determination of lamotrigine, oxcarbazepine monohydroxy derivative and felbamate in plasma of patients with epilepsy

A very simple and fast method has been developed and validated for simultaneous determination of the new generation antiepileptic drugs (AEDs) lamotrigine (LTG), oxcarbazepine's (OXC) main active metabolite monohydroxycarbamazepine and felbamate in plasma of patients with epilepsy using high-pe...

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Bibliographic Details
Published in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 828; no. 1; pp. 113 - 117
Main Authors:	Contin, Manuela, Balboni, Monica, Callegati, Erica, Candela, Carmina, Albani, Fiorenzo, Riva, Roberto, Baruzzi, Agostino
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 15-12-2005 Elsevier Science
Subjects:	Analysis Analytical, structural and metabolic biochemistry Anticonvulsants - blood Biological and medical sciences Carbamazepine - analogs & derivatives Carbamazepine - analysis Carbamazepine - blood Chromatography, High Pressure Liquid - methods Dibenzazepines - blood Drug Monitoring Epilepsy - blood Felbamate Fundamental and applied biological sciences. Psychology General pharmacology HPLC analysis Humans Lamotrigine Medical sciences Oxcarbazepine Oxcarbazepine main active metabolite Pharmacology. Drug treatments Phenylcarbamates Propylene Glycols - blood Spectrophotometry, Ultraviolet Therapeutic drug monitoring Triazines - blood Therapeutic drug monitoring Oxcarbazepine main active metabolite Felbamate HPLC analysis Lamotrigine Human Biological fluid Nervous system diseases Metabolite Epilepsy HPLC chromatography Anticonvulsant Liquid chromatography Cerebral disorder Blood plasma Oxcarbazepine Central nervous system disease Triazine derivatives Simultaneous measurement
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Summary:	A very simple and fast method has been developed and validated for simultaneous determination of the new generation antiepileptic drugs (AEDs) lamotrigine (LTG), oxcarbazepine's (OXC) main active metabolite monohydroxycarbamazepine and felbamate in plasma of patients with epilepsy using high-performance liquid chromatography (HPLC) with spectrophotometric detection. Plasma sample (500 μL) pre-treatment was based on simple deproteinization by acetonitrile. Liquid chromatographic analysis was carried out on a Synergi 4 μm Hydro-RP, 150 mm × 4 mm I.D. column, using a mixture of potassium dihydrogen phosphate buffer (50 mM, pH 4.5) and acetonitrile/methanol (3/1) (65:35, v/v) as the mobile phase, at a flow rate of 1.0 mL/min. The UV detector was set at 210 nm. Calibration curves were linear (mean correlation coefficient >0.999 for all the three analytes) over a range of 1–20 μg/mL for lamotrigine, 2–40 μg/mL for monohydroxycarbamazepine and 10–120 μg/mL for felbamate. Both intra and interassay precision and accuracy were lower than 7.5% for all three analytes. Absolute recoveries ranged between 100 and 104%. The present procedure describes for the first time the simultaneous determination of these three new antiepileptic drugs. The simple sample pre-treatment, combined with the fast chromatographic run permit rapid processing of a large series of patient samples.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2005.09.009