Host-controlled modification and restriction as a criterion of evaluating the therapeutical potential of Pseudomonas phage

The recently isolated phages phi ST3 and phi ST1 were compared as to their lysis behaviour in about 100 different P. aeruginosa strains. The growth of phi ST3 varies greatly in different host strains. We demonstrated one case of "non-classical", host-dependent modification and restriction....

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Bibliographic Details
Published in:	Journal of basic microbiology Vol. 31; no. 2; p. 101
Main Authors:	Gachechiladze, K K, Balardshishvili, N S, Adamia, R S, Chanishvili, T G, Krüger, D H
Format:	Journal Article
Language:	English
Published:	Germany 1991
Subjects:	Bacteriophages - genetics Bacteriophages - growth & development DNA, Viral - analysis Pseudomonas Infections - therapy
Online Access:	Get more information
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Summary:	The recently isolated phages phi ST3 and phi ST1 were compared as to their lysis behaviour in about 100 different P. aeruginosa strains. The growth of phi ST3 varies greatly in different host strains. We demonstrated one case of "non-classical", host-dependent modification and restriction. Here the capability to adsorb, and consequently to reproduce in a given host strain differs, depending on which modification the phage acquired in its former host. The DNA-containing phage phi ST1 displays stable lysis properties in the majority of the host strains. This makes phi ST1 a candidate for therapeutic phage preparations. One of the reasons for stable lysis properties is the apparent selection against recognition sites of restriction enzymes in its genome.
ISSN:	0233-111X
DOI:	10.1002/jobm.3620310206