Protective efficacy of IgA monoclonal antibodies to O and H antigens in a mouse model of intranasal challenge with Salmonella enterica serotype Enteritidis

Protective properties of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis ( S. enteritidis) were evaluated in a model of generalized infection after intranasal (i.n.) inoculation of BALB/c mice. Passive i.n. instillatio...

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Bibliographic Details
Published in:	Microbes and infection Vol. 6; no. 10; pp. 901 - 910
Main Authors:	Iankov, Ianko D., Petrov, Dragomir P., Mladenov, Ivan V., Haralambieva, Iana H., Kalev, Ognian K., Balabanova, Maria S., Mitov, Ivan G.
Format:	Journal Article
Language:	English
Published:	Lausanne Elsevier SAS 01-08-2004 Amsterdam Elsevier Paris
Subjects:	Animals Antibodies, Bacterial - immunology Antibodies, Bacterial - pharmacology Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacology Antigens, Bacterial - immunology Bacteriology Biological and medical sciences Disease Models, Animal Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology IgA Immunization, Passive - methods Immunoglobulin A - immunology Immunoglobulin A - pharmacology Intranasal infection Kinetics Lung Diseases - immunology Lung Diseases - microbiology Lung Diseases - prevention & control Mice Mice, Inbred BALB C Microbiology Miscellaneous Monoclonal antibodies O Antigens - immunology Protection Salmonella enterica Salmonella enteritidis Salmonella enteritidis - immunology Salmonella Infections, Animal - immunology Salmonella Infections, Animal - microbiology Salmonella Infections, Animal - prevention & control Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Salmonella enteritidis PBS MAbs IgA i.g i.n s.c i.p LD 50 LPS S-IgA MAb i.v DMEM CFU FBS Intranasal infection Monoclonal antibodies Protection Animal model Intranasal administration Monoclonal antibody Infection Prevention Immunoprophylaxis O antigen Efficiency Viral disease Bacteria Passive immunization Enterobacteriaceae
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Summary:	Protective properties of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis ( S. enteritidis) were evaluated in a model of generalized infection after intranasal (i.n.) inoculation of BALB/c mice. Passive i.n. instillation of antibodies 1 h before i.n. challenge did not prevent infection, and mice developed rapid inflammatory response in the lower respiratory tract. The passive systemic immunization was partially protective and a single intravenous (i.v.) injection of both O and H antigen specific IgA antibodies prolonged survival period of the infected animals. Permanent secretion of O:9 specific IgA MAb 177E6 into the respiratory tract in a “backpack” tumor model protected 50% of animals infected i.n. with a high dose of virulent S. enteritidis strain. Thus, secretory IgA (S-IgA) directed against O:9 antigen alone can prevent bacterial invasion in the respiratory epithelium.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1286-4579 1769-714X
DOI:	10.1016/j.micinf.2004.05.007