PEG-fibrin conjugates: the PEG impact on the polymerization dynamics

Fibrin and its modifications, particularly those with functionalized polyethylene glycol (PEG), remain highly attractive as a biomaterial in drug delivery and regenerative medicine. Despite the extensive knowledge of fibrinogenesis, there is little information on the processes occurring after its mo...

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Bibliographic Details
Published in:	Soft matter Vol. 19; no. 13; p. 2430
Main Authors:	Bakulina, Alesia A, Musina, Guzel R, Gavdush, Arsenii A, Efremov, Yuri M, Komandin, Gennady A, Vosough, Massoud, Shpichka, Anastasia I, Zaytsev, Kirill I, Timashev, Peter S
Format:	Journal Article
Language:	English
Published:	England 29-03-2023
Subjects:	Biocompatible Materials - chemistry Fibrin - chemistry Polyethylene Glycols - chemistry Polymerization Regenerative Medicine
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Summary:	Fibrin and its modifications, particularly those with functionalized polyethylene glycol (PEG), remain highly attractive as a biomaterial in drug delivery and regenerative medicine. Despite the extensive knowledge of fibrinogenesis, there is little information on the processes occurring after its modification. Previously, we found structural differences between native fibrin and its conjugates with PEG that allows us to hypothesize that a combination of methods such as terahertz (THz) pulsed spectroscopy and rheology may contribute to the characterization of gelation and reveal the effect of PEG on the polymerization dynamics. Compared to native fibrin, PEGylated fibrins had a homogenously soft surface; PEGylation also led to a significant decrease in the gelation time: from 42.75 min for native fibrin to 31.26 min and 35.09 min for 5 : 1 and 10 : 1 PEGylated fibrin, respectively. It is worth noting that THz pulsed spectroscopy makes it possible to reliably investigate only the polymerization process itself, while it does not allow us to observe statistically significant differences between the distinct PEGylated fibrin gels. The polymerization time constant of native fibrin measured by THz pulsed spectroscopy was 14.4 ± 2.8 min. However, it could not be calculated for PEGylated fibrin because the structural changes were too rapid. These results, together with those previously reported, led us to speculate that PEG-fibrin conjugates formed homogenously distributed highly water-shelled aggregates without bundling compared to native fibrin, ensuring rapid gelation and stabilization of the system without increasing its complexity.
ISSN:	1744-6848
DOI:	10.1039/d2sm01504h