Rapid Hydrolysis and Derivatization of Nitrofuran Metabolites with a New Derivatizing Agent 5-Nitro-2-Furaldehyde in Their Determination in Chicken Meat by HPLC–MS/MS

Rapid Hydrolysis and Derivatization of Nitrofuran Metabolites with a New Derivatizing Agent 5-Nitro-2-Furaldehyde in Their Determination in Chicken Meat by HPLC–MS/MS

We proposed a procedure for the rapid sample preparation of food samples for the determination of nitrofuran metabolites in them by HPLC–MS/MS, using 5-nitro-2-furaldehyde as a new derivatizing agent. Meat samples from broiler chickens treated with nitrofurans for 10 days were used to demonstrate th...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) Vol. 77; no. 10; pp. 1300 - 1306
Main Authors: Melekhin, A. O., Tolmacheva, V. V., Kholyavskaya, Yu. N., Sedykh, E. S., Dmitrienko, S. G., Apyari, V. V., Bairov, A. L.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-10-2022
Springer
Springer Nature B.V
Subjects:
Analysis
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chickens
Furans
High performance liquid chromatography
Hydrochloric acid
Hydrolysis
Mass spectrometry
Meat
Metabolites
Methods
Nitrogen compounds
Russia
nitrofuran metabolites
hypercrosslinked polystyrene
chicken meat analysis
HPLC with mass spectrometric detection
solid-phase extraction
5-nitro-2-furaldehyde
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Summary:We proposed a procedure for the rapid sample preparation of food samples for the determination of nitrofuran metabolites in them by HPLC–MS/MS, using 5-nitro-2-furaldehyde as a new derivatizing agent. Meat samples from broiler chickens treated with nitrofurans for 10 days were used to demonstrate that the time of hydrolysis–derivatization in a thermostated ultrasonic bath could be significantly shortened by raising temperature to 80°C and increasing the concentration of hydrochloric acid to 0.5 M. Metabolites of Furaltadone, Furazolidone, Nitrofurantoin, and Nitrofurazone in chicken meat, liver, stomach, and heart are determined using the developed procedure and an official method. The proposed method ensures the quantitative isolation of metabolites.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934822100112