Morphological and behavioral changes in the pathogenesis of a novel mouse model of communicating hydrocephalus

The Ro1 model of hydrocephalus represents an excellent model for studying the pathogenesis of hydrocephalus due to its complete penetrance and inducibility, enabling the investigation of the earliest cellular and histological changes in hydrocephalus prior to overt pathology. Hematoxylin and eosin s...

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Bibliographic Details
Published in:	PloS one Vol. 7; no. 1; p. e30159
Main Authors:	McMullen, Allison B, Baidwan, Gurlal S, McCarthy, Ken D
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 24-01-2012 Public Library of Science (PLoS)
Subjects:	Analysis Animal cognition Animals Aquaporins Behavior Behavior, Animal - physiology Biology Brain Brain - pathology Brain - physiopathology Brain - ultrastructure Cardiomegaly - pathology Cerebral Aqueduct - pathology Cerebral Aqueduct - ultrastructure Cerebral Ventricles - pathology Cerebral Ventricles - ultrastructure Cilia Cognitive development Communication Congenital diseases Denudation Disease Models, Animal Edema Electron microscopy Hydrocephalus Hydrocephalus - complications Hydrocephalus - genetics Hydrocephalus - pathology Hydrocephalus - physiopathology Immunofluorescence Laboratory animals Learning disabilities Medicine Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Electron Nervous system Pathogenesis Pharmacology Receptors, Opioid, kappa - genetics Receptors, Opioid, kappa - metabolism Receptors, Opioid, kappa - physiology Rodents Spina bifida Stem cells Studies Ventricle North Carolina United States > US
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Summary:	The Ro1 model of hydrocephalus represents an excellent model for studying the pathogenesis of hydrocephalus due to its complete penetrance and inducibility, enabling the investigation of the earliest cellular and histological changes in hydrocephalus prior to overt pathology. Hematoxylin and eosin staining, immunofluorescence and electron microscopy were used to characterize the histopathological events of hydrocephalus in this model. Additionally, a broad battery of behavioral tests was used to investigate behavioral changes in the Ro1 model of hydrocephalus. The earliest histological changes observed in this model were ventriculomegaly and disorganization of the ependymal lining of the aqueduct of Sylvius, which occurred concomitantly. Ventriculomegaly led to thinning of the ependyma, which was associated with periventricular edema and areas of the ventricular wall void of cilia and microvilli. Ependymal denudation was subsequent to severe ventriculomegaly, suggesting that it is an effect, rather than a cause, of hydrocephalus in the Ro1 model. Additionally, there was no closure of the aqueduct of Sylvius or any blockages within the ventricular system, even with severe ventriculomegaly, suggesting that the Ro1 model represents a model of communicating hydrocephalus. Interestingly, even with severe ventriculomegaly, there were no behavioral changes, suggesting that the brain is able to compensate for the structural changes that occur in the pathogenesis of hydrocephalus if the disorder progresses at a sufficiently slow rate.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: ABM KDM. Performed the experiments: ABM GSB. Analyzed the data: ABM. Wrote the paper: ABM KDM.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0030159