Ultrasensitive serological digoxin fluorescence sensor based on cascade amplification of entropy-driven DNA nanomachine and proximity ligation assay

Ultrasensitive serological digoxin fluorescence sensor based on cascade amplification of entropy-driven DNA nanomachine and proximity ligation assay

[Display omitted] •Fabrication of cyclically amplifying DNA nanomachine.•Fluorescence signal detection through proximity ligation system conjugated with qPCR.•Low detection limits of 0.06 ng/ml.•A good linear relationship with DIG concentrations during the detection range of 0.1 ∼ 5 ng/ml.•Selective...

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Bibliographic Details
Published in:Microchemical journal Vol. 200; p. 110452
Main Authors: Li, Keyu, Xiao, Peng, Wang, Chuning, Zhang, Zhilun, Feng, Jiaxi, Cai, Yijia, Xiang, Yixiao, Bai, Xuelu, Zuo, Guowei, Han, Baoru
Format: Journal Article
Language:English
Published: Elsevier B.V 01-05-2024
Subjects:
Aptamer
Digoxin
DNA nanomachine
Fluorescence
Proximity ligation assay
Toehold-mediated strand displacement
Fluorescence
Proximity ligation assay
Aptamer
DNA nanomachine
Digoxin
Toehold-mediated strand displacement
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Summary:[Display omitted] •Fabrication of cyclically amplifying DNA nanomachine.•Fluorescence signal detection through proximity ligation system conjugated with qPCR.•Low detection limits of 0.06 ng/ml.•A good linear relationship with DIG concentrations during the detection range of 0.1 ∼ 5 ng/ml.•Selective DIG detection in human blood serum. A nanobiosensor was prepared by DNA nanomachine based on proximity ligation assay (PLA) for the detection of digoxin (DIG) in serum. In this sensor the DNA nanomachine releases a large number of signal probes (SP) after addition of DIG, which participate in PLA as the connector strand, initiating real-time quantitative polymerase chain reaction (qPCR) and producing fluorescence signals. For the benefit of clinical therapeutics, we determined the detection range of 0.1 ∼ 5 ng/mL. Under the optimal experimental conditions, the cycle threshold (Ct) value showed a good linear relationship with DIG concentrations, and the detection limit was as low as 0.06 ng/mL (3σ). This method achieved the ultrasensitive detection of DIG by signal amplification and was successfully used for the detection of DIG in real serum, which has a good potential for application in the field of therapeutic drug monitoring.
ISSN:0026-265X
1095-9149
DOI:10.1016/j.microc.2024.110452