Search Results - "Bai, Wenqin"
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Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk
Published in Biomacromolecules (10-09-2018)“…Despite significant efforts to engineer their heterologous production, recombinant spider silk proteins (spidroins) have yet to replicate the unparalleled…”
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BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus
Published in Microbial cell factories (09-08-2024)“…To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize…”
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3
Engineering of a Highly Efficient Escherichia coli Strain for Mevalonate Fermentation through Chromosomal Integration
Published in Applied and environmental microbiology (15-12-2016)“…Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra…”
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4
Covalently-assembled single-chain protein nanostructures with ultra-high stability
Published in Nature communications (25-07-2019)“…Protein nanostructures with precisely defined geometries have many potential applications in catalysis, sensing, signal processing, and drug delivery. While…”
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Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid
Published in Microbial cell factories (29-12-2022)“…Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. For glutamic acid-independent strains, the titer of γ-PGA is too low to meet…”
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Structural Insight into and Mutational Analysis of Family 11 Xylanases: Implications for Mechanisms of Higher pH Catalytic Adaptation
Published in PloS one (10-07-2015)“…To understand the molecular basis of higher pH catalytic adaptation of family 11 xylanases, we compared the structures of alkaline, neutral, and acidic active…”
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Identification, systematic evolution and expression analyses of the AAAP gene family in Capsicum annuum
Published in BMC genomics (22-06-2021)“…Abstract Background The amino acid/auxin permease (AAAP) family represents a class of proteins that transport amino acids across cell membranes. Members of…”
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A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing
Published in BMC biotechnology (10-01-2024)“…Lytic polysaccharide monooxygenases (LPMOs) catalyzing the oxidative cleavage of different types of polysaccharides have potential to be used in various…”
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High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06
Published in Frontiers in bioengineering and biotechnology (26-01-2023)“…Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of…”
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Global Analysis of Gene Expression Profiles in Glutinous Rice 89-1 (Oryza sativa L.) Seedlings Exposed to Chilling Stress
Published in Plant molecular biology reporter (01-09-2021)“…Rice ( Oryza sativa ) is a tropical cereal crop that is severely affected by chilling stress at the seedling stage, although glutinous rice 89-1 (Gr89-1) in…”
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Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis
Published in BMC biotechnology (08-11-2016)“…Family 11 alkaline xylanases have great potential economic applications in the pulp and paper industry. In this study, we would improve the alkalophilicity of…”
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12
OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival
Published in Rice (15-04-2020)“…Background The large family of pentatricopeptide repeat (PPR) proteins is widely distributed among land plants. Such proteins play vital roles in intron…”
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A novel photocurable pullulan-based bioink for digital light processing 3D printing
Published in International journal of bioprinting (01-01-2023)“…Digital light processing has significant advantages, such as high repeatability, low failure rate, and no extrusion shear force. To make it possible to print…”
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14
Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp. SN5
Published in Biotechnology letters (01-11-2012)“…A xylanase gene (xyn10A) was cloned from Bacillus sp. SN5 and expressed in Escherichia coli. It encoded a 348-residue polypeptide of ~45 kDa. The deduced amino…”
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Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli
Published in Metabolic engineering (01-11-2016)“…Dicarboxylic acids are attractive biosynthetic targets due to their broad applications and their challenging manufacturing process from fossil fuel feedstock…”
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Combined Metabolomics and Network Pharmacology Analysis Reveal the Effect of Rootstocks on Anthocyanins, Lipids, and Potential Pharmacological Ingredients of Tarroco Blood Orange ( Citrus sinensis L. Osbeck)
Published in Plants (Basel) (14-08-2024)“…The benefits of citrus fruits are strongly associated with their secondary metabolites. In this study, we conducted widely targeted metabolomics analyses to…”
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Extracellular Overexpression of a Neutral Pullulanase in Bacillus subtilis through Multiple Copy Genome Integration and Atypical Secretion Pathway Enhancement
Published in Bioengineering (Basel) (28-06-2024)“…Neutral pullulanases, having a good application prospect in trehalose production, showed a limited expression level. In order to address this issue, two…”
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N-Terminal Sequences of Signal Peptides Assuming Critical Roles in Expression of Heterologous Proteins in Bacillus subtilis
Published in Microorganisms (Basel) (23-06-2024)“…The N-terminal sequences of proteins and their corresponding encoding sequences may play crucial roles in the heterologous expression. In this study, the…”
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Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an Engineered Bacillus subtilis Strain
Published in Bioengineering (Basel) (18-08-2023)“…At present, the double-enzyme catalyzed method using maltooligosyltrehalose synthase (MTSase) and maltooligosyltrehalose trehalohydrolase (MTHase) is the…”
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Overexpression of a Thermostable α-Amylase through Genome Integration in Bacillus subtilis
Published in Fermentation (Basel) (01-01-2023)“…A carbohydrate binding module 68 (CBM68) of pullulanase from Anoxybacillus sp. LM18-11 was used to enhance the secretory expression of a thermostable α-amylase…”
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