Diffracted X-ray blinking measurements of interleukin 15 receptors in the inner/outer membrane of living NK cells
Interleukin 15 receptor (IL-15R) is a transmembrane signalling protein consisting of 3 subsets: α, β (IL-15Rβ), and γ (γc). IL-2 and IL-15 share the signalling domains IL-15Rβ and γc, although they bind to intrinsic α-subsets and non-signalling domains. Additionally, IL-2 and IL-15 play different ro...
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| Published in: | Biochemical and biophysical research communications Vol. 556; pp. 53 - 58 |
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| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Elsevier Inc
04-06-2021
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Interleukin 15 receptor (IL-15R) is a transmembrane signalling protein consisting of 3 subsets: α, β (IL-15Rβ), and γ (γc). IL-2 and IL-15 share the signalling domains IL-15Rβ and γc, although they bind to intrinsic α-subsets and non-signalling domains. Additionally, IL-2 and IL-15 play different roles; therefore, there have been many observations of the dynamic behaviours of IL-15R, which are linked to physiological functions.
For more practical discrimination between IL-2 and IL-15, a study was designed and carried out in which α-subsets were removed and a cytoplasmic inhibitor was applied to create a simplified environment in which secondary signalling molecules were reduced. We also applied a new measurement method, diffracted X-ray blinking (DXB), to achieve higher accuracy (<0.01 Å).
The dynamics of IL-2 binding (confined motion, max range = 0.71 Å) and IL-15 binding (normal motion) in live natural killer cells were different. We also confirmed.
that DXB was a suitable method to quantitatively evaluate the transmembrane protein dynamics of inner/outer live cell membranes by labeling the extracellular domain since the measurements were dependent on the cytosolic environment.
•Intramolecular dynamics of IL-2 and IL-15 with sharing receptor were discriminated quantitatively in the live NK cell.•IL-2 binding narrowed the distance between β and γ domain, and changed the diffusion mode in the presence of nifuroxazide.•Diffracted X-ray Blinking (DXB) can determine the functional motion of inner/outer transmembrane proteins. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/j.bbrc.2021.03.144 |