Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry

Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry

In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-S...

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Bibliographic Details
Published in:Mass spectrometry letters Vol. 9; no. 2; pp. 56 - 60
Main Authors: Song, Insu, Lee, Jae-ung, Baek, Jaehyeon, Cha, Sangwon, Han, Sang Yun, Oh, Han Bin
Format: Journal Article
Language:English
Korean
Published: 한국질량분석학회 01-06-2018
사단법인 한국질량분석학회
Subjects:
화학
FRIPS
radical ion
MALDI-TOF/TOF
mass spectrometry
peptides
radical-driven fragmentation
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Summary:In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly $[a_n+H]^+$, $[z_n+H]^+$, and $[y_n]^+-type$ products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.
Bibliography:KISTI1.1003/JNL.JAKO201821464987776
ISSN:2233-4203
2093-8950
DOI:10.5478/MSL.2018.9.2.56