Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertus...

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Bibliographic Details
Published in:Infection and Immunity Vol. 67; no. 8; pp. 3763 - 3767
Main Authors: Preston, A, Allen, A.G, Cadisch, J, Thomas, R, Stevens, K, Churcher, C.M, Badcock, K.L, Parkhill, J, Barrell, B, Maskell, D.J
Format: Journal Article
Language:English
Published: Washington, DC American Society for Microbiology 01-08-1999
Subjects:
bacterial antigens
Bacteriology
Biological and medical sciences
Bordetella - immunology
Bordetella bronchiseptica
Bordetella bronchiseptica - immunology
Bordetella parapertussis
Bordetella pertussis
Chromosome Mapping
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
genbank/aj007747
Genetics
loci
Microbiology
Molecular and Cellular Pathogenesis
molecular sequence data
Mutation
nucleotide sequences
O Antigens - biosynthesis
Open Reading Frames
Characterization
Bordetella bronchiseptica
O antigen
Gene
Bordetella parapertussis
Lipopolysaccharide
Bacteria
Biosynthesis
Bordetella pertussis
Locus
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Description
Summary:Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.
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Corresponding author. Mailing address: Centre for Veterinary Medicine, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom. Phone: 44 1223 766233. Fax: 44 1223 337610. E-mail: ap243@mole.bio.cam.ac.uk.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.67.8.3763-3767.1999