Characterization of a Chitinase (Chit62) from Serratia marcescens B4A and Its Efficacy as a Bioshield Against Plant Fungal Pathogens

Chitinases have been suggested to be involved in pathogen–antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity a...

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Bibliographic Details
Published in:	Biochemical genetics Vol. 50; no. 9-10; pp. 722 - 735
Main Authors:	Babashpour, S, Aminzadeh, S, Farrokhi, N, Karkhane, A, Haghbeen, K
Format:	Journal Article
Language:	English
Published:	Boston Springer-Verlag 01-10-2012 Springer US Springer Nature B.V
Subjects:	Alternaria - drug effects Alternaria - growth & development Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - pharmacology Biochemistry Biological control Biological Products - isolation & purification Biological Products - pharmacology Biomedical and Life Sciences Biomedicine Chitin - metabolism chitinase Chitinases - genetics Chitinases - isolation & purification Chitinases - pharmacology Computational Biology DNA, Bacterial Electrophoresis, Polyacrylamide Gel Enzymatic activity Enzyme Activation enzyme activity Enzyme Assays Escherichia coli - genetics Escherichia coli - metabolism Fusarium - drug effects Fusarium - growth & development Human Genetics Hydrogen-Ion Concentration Medical Microbiology Microbial Sensitivity Tests Mycelium - drug effects Mycelium - growth & development Pathogens Phylogeny plant pathogenic fungi Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - pharmacology Rhizoctonia - drug effects Rhizoctonia - growth & development Serratia marcescens Serratia marcescens - enzymology Serratia marcescens - genetics Temperature Zoology Chitinase Fungal biocontrol Protein activity assay Heterologous expression
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Summary:	Chitinases have been suggested to be involved in pathogen–antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters. Active enzyme was used to determine its biocontrol features against fungal phytopathogens. The results demonstrated that the optimum pH and temperature for the enzyme activity were 6.0 and 55 °C, respectively. The K m and V max values were 3.30 mg ml−1 and 0.92 units, respectively. The recombinant chitinase was demonstrated to be highly active in controlling fungal pathogens.
Bibliography:	http://dx.doi.org/10.1007/s10528-012-9515-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-2928 1573-4927
DOI:	10.1007/s10528-012-9515-3