Search Results - "BRAKENHOFF, G. J."

Image calibration in fluorescence microscopy by ZWIER, J. M., VAN ROOIJ, G. J., HOFSTRAAT, J. W., BRAKENHOFF, G. J.

“…Summary A fluorescence image calibration method is presented based on the use of standardized uniformly fluorescing reference layers. It is demonstrated to be…”
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Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy by MANDERS, E. M. M, STAP, J, BRAKENHOFF, G. J, VAN DRIEL, R, ATEN, J. A

“…The temporal and spatial progression of DNA replication in interphase nuclei of eukaryotic cells has been investigated. Application of a recently developed…”
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Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation by Pillai, Rajesh S, Brakenhoff, G J, Müller, M

“…The third-order nonlinear susceptibility (chi(3)) can be measured quantitatively using third-harmonic generation (THG) from two different interfaces. For the…”
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Characterization of sectioning fluorescence microscopy with thin uniform fluorescent layers: Sectioned Imaging Property or SIPcharts by BRAKENHOFF, G. J., WURPEL, G. W. H., JALINK, K., OOMEN, L., BROCKS, L., ZWIER, J. M.

“…Summary Thin, uniformly fluorescing reference layers can be used to characterize the imaging conditions in confocal, or more general, sectioning microscopy…”
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Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system by Brakenhoff, G J, Squier, J, Norris, T, Bliton, A C, Wade, M H, Athey, B

“…The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that…”
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A new method to study shape recovery of red blood cells using multiple optical trapping by Bronkhorst, P.J., Streekstra, G.J., Grimbergen, J., Nijhof, E.J., Sixma, J.J., Brakenhoff, G.J.

“…In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell…”
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The mechanism of red cell (dis)aggregation investigated by means of direct cell manipulation using multiple optical trapping by BRONKHORST, P. J. H., GRIMBERGEN, J., BRAKENHOFF, G. J., HEETHAAR, R. M., SIXMA, J. J.

“…We used multiple optical trapping to study the mechanism of red cell (dis)aggregation. Two sets of optical ‘tweezers’ were used to bring two red blood cells…”
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Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I: Intracellular localization during interphase by VERHEIJEN, R, KUIJPERS, H. J. H, SCHLINGEMANN, R. O, BOEHMER, A. L. M, VAN DRIEL, R, BRAKENHOFF, G. J, RAMAEKERS, F. C. S

“…Ki-67 is a commercially available mouse monoclonal antibody, which reacts with a nuclear antigen in proliferating cells. The antibody can be used to determine…”
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Four-dimensional imaging of chromatin dynamics during the assembly of the interphase nucleus by Manders, E M M, Visser, A E, Koppen, A, de Leeuw, W C, van Liere, R, Brakenhoff, G J, van Driel, R

“…Large-scale chromatin organization is likely to play an important role in epigenetic control of gene expression. This implies that after mitosis the correct…”
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Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II: Localization in mitotic cells and association with chromosomes by VERHEIJEN, R, KUIJPERS, H. J. H, VAN DRIEL, R, BECK, J. L. M, VAN DIERENDONCK, J. H, BRAKENHOFF, G. J, RAMAEKERS, F. C. S

“…In interphase cells the proliferation-associated antigen recognized by monoclonal antibody Ki-67 is almost exclusively located in the nucleoli. When cells at…”
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Optical far‐field microscopy of single molecules with 3.4 nm lateral resolution by Bloeß, A., Durand, Y., Matsushita, M., Van Dermeer, H., Brakenhoff, G. J., Schmidt, J.

“…Summary Optical far‐field imaging of single molecules in a frozen solution at 1.2 K with a lateral resolution of 3.4 nm is reported. The mechanical stability…”
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Imaging colloidal particle induced topological defects in a nematic liquid crystal using third harmonic generation microscopy by Pillai, Rajesh S, Oh-E, Masahito, Yokoyama, Hiroshi, Brakenhoff, G J, Müller, Michiel

“…The nature of the third-harmonic generation (THG) process in a nematic liquid crystal is investigated for the case of tightly focused, low intensity, laser…”
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Third harmonic generation microscopy by Squier, J, Muller, M, Brakenhoff, G, Wilson, K R

“…Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 aem results in a…”
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Analysis of efficiency of two-photon versus single-photon absorption of fluorescence generation in biological objects by Brakenhoff, G J, Müller, M, Ghauharali, R I

“…Starting from basic absorption cross-section values and breakdown limitations on possible field strengths it is shown that the total exposure required for…”
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3D microscopy of transparent objects using third‐harmonic generation by Müller, Squier, Wilson, Brakenhoff

“…It is demonstrated that third‐harmonic generation (THG) near interfaces in the refractive index or the third‐order nonlinear susceptibility (χ(3)) permits…”
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Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy by Brakenhoff, G. J, van der Voort, H. T. M, van Spronsen, E. A, Linnemans, W. A. M, Nanninga, N

“…The relationship between cell shape and function has long been of interest. However, although the behaviour of the cytoskeleton during the cell cycle has been…”
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Topography of Peptidoglycan Synthesis during Elongation and Polar Cap Formation in a Cell Division Mutant of Escherichia coli MC4100 by WOLDRINGH, C. L, HULS, P, PAS, E, BRAKENHOFF, G. J, NANNINGA, N

“…Department of Electron Microscopy and Molecular Cytology, University of Amsterdam, Plantage Muidergracht 14, 1018 TV Amsterdam, The Netherlands ABSTRACT…”
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Image cytometric method for quantifying the relative amount of DNA in bacterial nucleoids using Escherichia coli by Vischer, N O, Huls, P G, Ghauharali, R I, Brakenhoff, G J, Nanninga, N, Woldringh, C L

“…An image cytometric method for quantifying integrated fluorescence was developed to measure the relative DNA contents of bacterial nucleoids. Image analysis…”
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Quantitative image correction and calibration for confocal fluorescence microscopy using thin reference layers and SIPchart-based calibration procedures by ZWIER, J.M, OOMEN, L, BROCKS, L, JALINK, K, BRAKENHOFF, G.J

“…The fluorescence intensity image of an axially integrated through-focus series of a thin standardized uniform fluorescent layer can be used for image intensity…”
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Characterization of femtosecond pulses focused with high numerical aperture optics using interferometric surface-third-harmonic generation by Squier, J.A, Fittinghoff, D.N, Barty, C.P.J, Wilson, K.R, Müller, M, Brakenhoff, G.J

“…We demonstrate that the interferometric surface-third-harmonic-generation autocorrelation function from the glass/air interface of a coverslip can be used…”
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