Search Results - "BRADY, Sean F"

Culture-independent discovery of natural products from soil metagenomes by Katz, Micah, Hover, Bradley M, Brady, Sean F

“…Bacterial natural products have proven to be invaluable starting points in the development of many currently used therapeutic agents. Unfortunately,…”
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Discovery of indolotryptoline antiproliferative agents by homology-guided metagenomic screening by Chang, Fang-Yuan, Brady, Sean F.

“…Natural product discovery by random screening of broth extracts derived from cultured bacteria often suffers from high rates of redundant isolation, making it…”
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Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites by Feng, Zhiyang, Kallifidas, Dimitris, Brady, Sean F

“…A single gram of soil is predicted to contain thousands of unique bacterial species. The majority of these species remain recalcitrant to standard culture…”
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Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters by Montiel, Daniel, Hahk-Soo Kang, Fang-Yuan Chang, Zachary Charlop-Powers, Sean F. Brady

“…Large-scale sequencing of prokaryotic (meta)genomic DNA suggests that most bacterial natural product gene clusters are not expressed under common laboratory…”
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Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist by Cohen, Louis J., Kang, Hahk-Soo, Chu, John, Huang, Yun-Han, Gordon, Emma A., Reddy, Boojala Vijay B., Ternei, Melinda A., Craig, Jeffrey W., Brady, Sean F.

“…The trillions of bacteria that make up the human microbiome are believed to encode functions that are important to human health; however, little is known about…”
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Discovery of MRSA active antibiotics using primary sequence from the human microbiome by Chu, John, Vila-Farres, Xavier, Inoyama, Daigo, Ternei, Melinda, Cohen, Louis J, Gordon, Emma A, Reddy, Boojala Vijay B, Charlop-Powers, Zachary, Zebroski, Henry A, Gallardo-Macias, Ricardo, Jaskowski, Mark, Satish, Shruthi, Park, Steven, Perlin, David S, Freundlich, Joel S, Brady, Sean F

“…The synthetic bioinformatic natural products (syn-BNPs) approach identifies putative natural products that are validated directly by independent synthesis. Its…”
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Uncovering the biosynthetic potential of rare metagenomic DNA using co-occurrence network analysis of targeted sequences by Libis, Vincent, Antonovsky, Niv, Zhang, Mengyin, Shang, Zhuo, Montiel, Daniel, Maniko, Jeffrey, Ternei, Melinda A., Calle, Paula Y., Lemetre, Christophe, Owen, Jeremy G., Brady, Sean F.

“…Sequencing of DNA extracted from environmental samples can provide key insights into the biosynthetic potential of uncultured bacteria. However, the high…”
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Lapcin, a potent dual topoisomerase I/II inhibitor discovered by soil metagenome guided total chemical synthesis by Wang, Zongqiang, Forelli, Nicholas, Hernandez, Yozen, Ternei, Melinda, Brady, Sean F.

“…In natural product discovery programs, the power of synthetic chemistry is often leveraged for the total synthesis and diversification of characterized…”
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Recent application of metagenomic approaches toward the discovery of antimicrobials and other bioactive small molecules by Banik, Jacob J, Brady, Sean F

“…Abstract Bacteria grown in pure culture have been the starting point for the discovery of many of the antibacterials now in use. Metagenomics, which utilizes…”
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Tetarimycin A, an MRSA-Active Antibiotic Identified through Induced Expression of Environmental DNA Gene Clusters by Kallifidas, Dimitris, Kang, Hahk-Soo, Brady, Sean F

“…The propagation of DNA extracted directly from environmental samples in laboratory-grown bacteria provides a means to study natural products encoded in the…”
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Chemical-biogeographic survey of secondary metabolism in soil by Charlop-Powers, Zachary, Owen, Jeremy G, Reddy, Boojala Vijay B, Ternei, Melinda A, Brady, Sean F

“…In this study, we compare biosynthetic gene richness and diversity of 96 soil microbiomes from diverse environments found throughout the southwestern and…”
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Multiplexed mobilization and expression of biosynthetic gene clusters by Libis, Vincent, MacIntyre, Logan W., Mehmood, Rabia, Guerrero, Liliana, Ternei, Melinda A., Antonovsky, Niv, Burian, Ján, Wang, Zongqiang, Brady, Sean F.

“…Bacterial genomes contain large reservoirs of biosynthetic gene clusters (BGCs) that are predicted to encode unexplored natural products. Heterologous…”
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Mapping gene clusters within arrayed metagenomic libraries to expand the structural diversity of biomedically relevant natural products by Owen, Jeremy G., Reddy, Boojala Vijay B., Ternei, Melinda A., Charlop-Powers, Zachary, Calle, Paula Y., Kim, Jeffrey H., Brady, Sean F.

“…Complex microbial ecosystems contain large reservoirs of unexplored biosynthetic diversity. Here we provide an experimental framework and data analysis tool to…”
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High-throughput retrieval of target sequences from complex clone libraries using CRISPRi by Burian, Ján, Libis, Vincent K., Hernandez, Yozen A., Guerrero-Porras, Liliana, Ternei, Melinda A., Brady, Sean F.

“…The capture of metagenomic DNA in large clone libraries provides the opportunity to study microbial diversity that is inaccessible using culture-dependent…”
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Multiplexed metagenome mining using short DNA sequence tags facilitates targeted discovery of epoxyketone proteasome inhibitors by Owen, Jeremy G., Charlop-Powers, Zachary, Smith, Alexandra G., Ternei, Melinda A., Calle, Paula Y., Reddy, Boojala Vijay B., Montiel, Daniel, Brady, Sean F.

“…In molecular evolutionary analyses, short DNA sequences are used to infer phylogenetic relationships among species. Here we apply this principle to the study…”
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Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism by Peek, James, Lilic, Mirjana, Montiel, Daniel, Milshteyn, Aleksandr, Woodworth, Ian, Biggins, John B., Ternei, Melinda A., Calle, Paula Y., Danziger, Michael, Warrier, Thulasi, Saito, Kohta, Braffman, Nathaniel, Fay, Allison, Glickman, Michael S., Darst, Seth A., Campbell, Elizabeth A., Brady, Sean F.

“…Rifamycin antibiotics (Rifs) target bacterial RNA polymerases (RNAPs) and are widely used to treat infections including tuberculosis. The utility of these…”
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Fluostatins Produced by the Heterologous Expression of a TAR Reassembled Environmental DNA Derived Type II PKS Gene Cluster by Feng, Zhiyang, Kim, Jeff H, Brady, Sean F

“…Culture independent approaches for accessing small molecules produced by uncultured bacteria are often hampered by the inability to easily clone environmental…”
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Cloning and characterization of new glycopeptide gene clusters found in an environmental DNA megalibrary by Banik, Jacob J, Brady, Sean F

“…Glycopeptide antibiotics have long served as drugs of last resort for the treatment of antibiotic-resistant Gram-positive bacterial infections. Resistance to…”
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Unraveling function and diversity of bacterial lectins in the human microbiome by Cohen, Louis J., Han, Sun M., Lau, Pearson, Guisado, Daniela, Liang, Yupu, Nakashige, Toshiki G., Ali, Thamina, Chiang, David, Rahman, Adeeb, Brady, Sean F.

“…The mechanisms by which commensal organisms affect human physiology remain poorly understood. Lectins are non-enzymatic carbohydrate binding proteins that all…”
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Targeted Metagenomics: Finding Rare Tryptophan Dimer Natural Products in the Environment by Chang, Fang-Yuan, Ternei, Melinda A, Calle, Paula Y, Brady, Sean F

“…Natural product discovery from environmental genomes (metagenomics) has largely been limited to the screening of existing environmental DNA (eDNA) libraries…”
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