Search Results - "BOEKE, J. D"

Yeast 2.0-connecting the dots in the construction of the world's first functional synthetic eukaryotic genome by Pretorius, I S, Boeke, J D

“…Historians of the future may well describe 2018 as the year that the world's first functional synthetic eukaryotic genome became a reality. Without the benefit…”
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Sir2-Dependent Activation of Acetyl-CoA Synthetase by Deacetylation of Active Lysine by Starai, V. J., Celic, I., Cole, R. N., Boeke, J. D., Escalante-Semerena, J. C.

“…Acetyl-coenzyme A (CoA) synthetase (Acs) is an enzyme central to metabolism in prokaryotes and eukaryotes. Acs synthesizes acetyl CoA from acetate, adenosine…”
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Human L1 retrotransposition: cis preference versus trans complementation by Wei, W, Gilbert, N, Ooi, S L, Lawler, J F, Ostertag, E M, Kazazian, H H, Boeke, J D, Moran, J V

“…Long interspersed nuclear elements (LINEs or L1s) comprise approximately 17% of human DNA; however, only about 60 of the approximately 400,000 L1s are mobile…”
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An unusual form of transcriptional silencing in yeast ribosomal DNA by Smith, J S, Boeke, J D

“…Generalized transcriptional repression of large chromosomal regions in Saccharomyces cerevisiae occurs at the silent mating loci and at telomeres and is…”
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Chemistry of Gene Silencing:  The Mechanism of NAD+-Dependent Deacetylation Reactions by Sauve, Anthony A, Celic, Ivana, Avalos, Jose, Deng, Haiteng, Boeke, Jef D, Schramm, Vern L

“…The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD+-dependent protein deacetylation. New peptide substrates, the reaction…”
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Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe by Keeney, J.B, Boeke, J.D

“…Homologous integration into the fission yeast Schizosaccharomyces pombe has not been well characterized. In this study, we have examined integration of…”
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A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae by Ooi, Siew Loon, Shoemaker, Daniel D., Boeke, Jef D.

“…We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in…”
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A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase by Starai, VJ, Takahashi, H, Boeke, JD, Escalante-Semerena, JC

“…The silent information regulator protein (Sir2) and its homologs (collectively known as sirtuins) are NAD +-dependent deacetylase enzymes involved in…”
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Reverse Two-Hybrid and One-Hybrid Systems to Detect Dissociation of Protein-Protein and DNA-Protein Interactions by Vidal, Marc, Brachmann, Rainer K., Fattaey, Ali, Harlow, Ed, Boeke, Jef D.

“…Macromolecular interactions define many biological phenomena. Although genetic methods are available to identify novel protein-protein and DNA-protein…”
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The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability by Brachmann, C B, Sherman, J M, Devine, S E, Cameron, E E, Pillus, L, Boeke, J D

“…Genomic silencing is a fundamental mechanism of transcriptional regulation, yet little is known about conserved mechanisms of silencing. We report here the…”
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Integration of the yeast retrotransposon Ty1 is targeted to regions upstream of genes transcribed by RNA polymerase III by Devine, S E, Boeke, J D

“…Retroviruses and their relatives, the LTR-containing retrotransposons, integrate newly replicated cDNA copies of their genomes into the genomes of their hosts…”
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Genetic Characterization of a Mammalian Protein-Protein Interaction Domain by Using a Yeast Reverse Two-Hybrid System by Vidal, Marc, Braun, Pascal, Chen, Elbert, Boeke, Jef D., Harlow, Ed

“…Many biological processes rely upon protein-protein interactions. Hence, detailed analysis of these interactions is critical for their understanding. Due to…”
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Dominant-Negative p53 Mutations Selected in Yeast Hit Cancer Hot Spots by Brachmann, Rainer K., Vidal, Marc, Boeke, Jef D.

“…Clinically important mutant p53 proteins may be tumorigenic through a dominant-negative mechanism or due to a gain-of-function. Examples for both hypotheses…”
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Hotspots for unselected Ty1 transposition events on yeast chromosome III are near tRNA genes and LTR sequences by Ji, H, Moore, D P, Blomberg, M A, Braiterman, L T, Voytas, D F, Natsoulis, G, Boeke, J D

“…A collection of yeast strains bearing single marked Ty1 insertions on chromosome III was generated. Over 100 such insertions were physically mapped by…”
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Transcription and reverse transcription of retrotransposons by BOEKE, J. D, CORCES, V. G

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A Human Nuclear Shuttling Protein That Interacts with Human Immunodeficiency Virus Type 1 Matrix Is Packaged into Virions by Gupta, K, Ott, D, Hope, T J, Siliciano, R F, Boeke, J D

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Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein by TRUEHEART, J, BOEKE, J. D, FINK, G. R

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Replication infidelity during a single cycle of Ty1 retrotransposition by Gabriel, A. (Rutgers University, Piscataway, NJ.), Willems, M, Mules, E.H, Boeke, J.D

“…Retroviruses undergo a high frequency of genetic alterations during the process of copying their RNA genomes. However, little is known about the replication…”
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A critical proteolytic cleavage site near the C terminus of the yeast retrotransposon Ty1 Gag protein by Merkulov, G V, Swiderek, K M, Brachmann, C B, Boeke, J D

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Human L1 element target‐primed reverse transcription in vitro by Cost, Gregory J., Feng, Qinghua, Jacquier, Alain, Boeke, Jef D.

“…L1 elements are ubiquitous human transposons that replicate via an RNA intermediate. We have reconstituted the initial stages of L1 element transposition in…”
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