Phenolic compounds from Virola venosa (Myristicaceae) and evaluation of their antioxidant and enzyme inhibition potential

ABSTRACT Virola venosa, popularly known in Brazil as ucuuba-da-mata, occurs naturally in the Amazon region and has potential to provide useful natural compounds, as already known for other Virola species. Therefore, the objective of this study was to determine the chemical composition of bark and le...

Full description

Saved in:

Bibliographic Details
Published in:	Acta amazonica Vol. 49; no. 1; pp. 48 - 53
Main Authors:	FERNANDES, Kamila Rangel Primo, BITTERCOURT, Paulo Senna, SOUZA, Afonso Duarte Leão de, SOUZA, Antonia Queiroz Lima de, SILVA, Felipe Moura Araújo da, LIMA, Emerson Silva, ACHO, Leonard Domingo Rosales, NUNOMURA, Rita de Cássia Saraiva, TEIXEIRA, Ana Frazão, KOOLEN, Hector Henrique Ferreira
Format:	Journal Article
Language:	English
Published:	Instituto Nacional de Pesquisas da Amazônia 01-03-2019
Subjects:	Amazon antioxidant compounds ferulic acid α-glucosidase inhibition
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	ABSTRACT Virola venosa, popularly known in Brazil as ucuuba-da-mata, occurs naturally in the Amazon region and has potential to provide useful natural compounds, as already known for other Virola species. Therefore, the objective of this study was to determine the chemical composition of bark and leaf extracts of V. venosa, and to test the antioxidant capacity and α-glucosidase inhibition potential of their compounds. Polar extracts showed to be more active in both assays, therefore a bioactivity-guided fractionation was performed to identify the compounds that were responsible for the recorded activities. Using a combination of LC-MS/MS analysis and isolation with NMR identification, eight phenolic compounds were identified. Assays with pure compounds of the active fraction revealed that ferulic acid was the main contributor compound to the observed bioactivity in the crude extracts. Resumo Virola venosa, popularmente conhecida como ucuuba-da-mata, ocorre naturalmente na região amazônica e tem potencial para fornecer compostos naturais úteis, como já foi mostrado para outras espécies de Virola. Por isso, o objetivo deste estudo foi determinar a composição química dos extratos do tronco e das folhas de V. venosa e os possíveis potenciais antioxidantes e de inibição contra α-glucosidase de seus compostos. Os extratos polares mostraram-se mais ativos em ambos os testes, portanto, um fracionamento guiado por bioatividade foi realizado para designar os compostos responsáveis pelas atividades registradas. Através da combinação de análise CL-EM/EM e isolamento com identificação por RMN, foram identificados oito compostos fenólicos. Testes com os compostos puros principais das frações mais ativas indicaram o ácido ferúlico como o principal contribuinte das atividades biológicas observadas para os extratos brutos, e, consequentemente, o princípio ativo principal de V. venosa.
ISSN:	0044-5967 1809-4392
DOI:	10.1590/1809-4392201800832