Enalapril-induced Apoptosis of Acute Promyelocytic Leukaemia Cells Involves STAT5A

In this study, we aimed at evaluating the cytotoxic and apoptotic effects of enalapril on human HL60 acute promyelocytic leukaemia cells and at clarifying the roles of signal transducers and activator of transcription proteins (STATs) on enalapril-induced cell death. Cell viability and cytotoxicity...

Full description

Saved in:

Bibliographic Details
Published in:	Anticancer research Vol. 32; no. 7; pp. 2885 - 2893
Main Authors:	PURCLUTEPE, Ozlem, ISKENDER, Guniz, SAYDAM, Guray, DEMET KIPER, Hatice, TEZCANLI, Burcin, SELVI, Nur, BIRAY AVCI, Cigir, KOSOVA, Buket, ADAN GOKBULUT, Aysun, SAHIN, Fahri, BARAN, Usuf
Format:	Journal Article
Language:	English
Published:	Attiki International Institute of Anticancer Research 01-07-2012
Subjects:	Angiotensin-Converting Enzyme Inhibitors - pharmacology Apoptosis Apoptosis - drug effects Biological and medical sciences Cell proliferation Cytotoxicity Data processing Dose-Response Relationship, Drug Enalapril - pharmacology Gene Expression - drug effects Green fluorescent protein Hematologic and hematopoietic diseases HL-60 Cells Humans Leukemia, Promyelocytic, Acute - drug therapy Leukemia, Promyelocytic, Acute - genetics Leukemia, Promyelocytic, Acute - metabolism Leukemia, Promyelocytic, Acute - pathology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Polymerase chain reaction Salts Stat3 protein STAT3 Transcription Factor - biosynthesis STAT3 Transcription Factor - genetics STAT5 Transcription Factor - biosynthesis STAT5 Transcription Factor - genetics Transcription Tumor Suppressor Proteins - biosynthesis Tumor Suppressor Proteins - genetics Tumors Promyelocytic leukemia Enzyme Acute STATs Enzyme inhibitor Cytotoxicity Malignant hemopathy Acute promyelocytic leukaemia HL60 cell line HL60 cells Peptidases Signal transduction Cancerology Enalapril Peptidyl-dipeptidase A Cell death Hydrolases Peptidyl-dipeptidases Transduction factor STAT ACE inhibitor Cancer Apoptosis M3 acute myelocytic leukemia
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	In this study, we aimed at evaluating the cytotoxic and apoptotic effects of enalapril on human HL60 acute promyelocytic leukaemia cells and at clarifying the roles of signal transducers and activator of transcription proteins (STATs) on enalapril-induced cell death. Cell viability and cytotoxicity tests were conducted by Trypan blue dye exclusion and 2,3-Bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide inner salt (XTT) assays, respectively. Apoptotic analyses were performed by the AnnexinV-enhanced green fluorescent protein (EGFP) staining method and by fluorescence microscopy. Expression levels of STAT3, -5A and -5B genes were analysed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The results showed that enalapril reduced viability and proliferation, and induced apoptosis in HL60 cells in a dose- and time-dependent manner as compared to untreated controls. The expression levels of STAT5A gene were significantly reduced in enalapril-treated HL60 cells as compared to untreated controls. Taken together, all data showed for the first time that enalapril has significant anticancer potential for the treatment of acute premyelocytic leukaemia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0250-7005 1791-7530