Soluble Components of Histoplasma Capsulatum var. Capsulatum have Hemagglutinin Activity and Induce Syngeneic Hemophagocytosis In Vitro

Soluble Components of Histoplasma Capsulatum var. Capsulatum have Hemagglutinin Activity and Induce Syngeneic Hemophagocytosis In Vitro

Histoplasma capsulatum var. capsulatum is a thermally dimorphic fungus that causes histoplasmosis. Fungal hemagglutination activity and cases of reactive hemophagocytic syndrome (RHS) have been reported in the disseminated form of disease. In the present study, soluble components of H. capsulatum va...

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Bibliographic Details
Published in:Mycopathologia (1975) Vol. 169; no. 3; pp. 151 - 157
Main Authors: Vivan, R. H. F, Leonello, P. C, Nagashima, L. A, Kaminami, M. S, Tristão, F. S. M, Sano, A, Ono, M. A, Béjar, C. V, Itano, E. N
Format: Journal Article
Language:English
Published: Dordrecht Dordrecht : Springer Netherlands 01-03-2010
Springer Netherlands
Springer Nature B.V
Subjects:
Animals
Antigens
Biomedical and Life Sciences
Enzyme-Linked Immunosorbent Assay
Eukaryotic Microbiology
Fungal Proteins - chemistry
Fungal Proteins - immunology
Fungal Proteins - isolation & purification
Fungi
Hemagglutination
Histoplasma - chemistry
Histoplasma capsulatum
Life Sciences
Macrophages, Peritoneal - immunology
Medical Microbiology
Mice
Mice, Inbred BALB C
Microbial Ecology
Microbiology
Molecular Weight
Phagocytosis
Plant Sciences
Polyclonal antibodies
Yeast
Brazil
St Louis Missouri
United States > US
Peru
Antibodies
Hemagglutination
Reactive hemophagocytic syndrome
Red blood cells
Disseminated histoplasmosis
ELISA
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Summary:Histoplasma capsulatum var. capsulatum is a thermally dimorphic fungus that causes histoplasmosis. Fungal hemagglutination activity and cases of reactive hemophagocytic syndrome (RHS) have been reported in the disseminated form of disease. In the present study, soluble components of H. capsulatum var. capsulatum have been investigated for hemagglutinin activity and the capacity to induce hemophagocytosis in the mouse system. To analyze hemagglutinating activity, mouse red blood cells (RBC) (1% v/v in PBS) were incubated (37°C, 1 h) with cell-free antigen (CFAg) from H. capsulatum var. capsulatum (isolate IMT/HC128) (RBC-CFAg) or previously heated CFAg (56°C, 30 min) (RBC-hCFAg) or as control with PBS (RBC-PBS). Hemophagocytosis was analyzed by incubating BALB/c mouse peritoneal phagocytic cells (5 × 10⁶ cells) with syngeneic RBC, sensitized or not with CFAg. In addition, mouse polyclonal antibodies were raised against syngeneic RBC-CFAg (anti-RBC-CFAg) and used to analyze CFAg chromatographic fractions (Sephadex G75/120) by immunoenzymatic assay (ELISA). Hemagglutinin activity was observed with RBC-CFAg, but not with RBC-hCFAg or RBC. Also, hemophagocytosis was observed with RBC-CFAg, but not with RBC. The anti-RBC-CFAg antibodies reacted with CFAg fractions corresponding to a molecular mass (MM) higher than 150 kDa. In conclusion, the yeast form of H. capsulatum var. capsulatum releases thermolabile soluble components with hemagglutinin activity and it has been demonstrated for the first time that soluble components of the same fungus induce syngeneic hemophagocytosis in the in vitro mouse system. Also, indirect analysis with antibodies suggests that high-MM components (>150 kDa) are responsible for the interaction with RBC.
Bibliography:http://dx.doi.org/10.1007/s11046-009-9243-3
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ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-009-9243-3