Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ

Pancreatic carcinoma is a very aggressive disease and little is known about its immunobiology. We here describe the presence in pancreatic cancer patients of spontaneously induced functional CD4 and CD8 memory/effector T cells reactive to autologous tumor cells or to the pancreatic cancer associated...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of oncology Vol. 28; no. 6; pp. 1419 - 1428
Main Authors:	SCHMITZ-WINNENTHAL, F. H, GALINDO ESCOBEDO, L. V, WEITZ, J, BÜCKLER, M. W, Z'GRAGGEN, K, BECKHOVE, P, SCHIRRMACHER, V, BUCUR, M, ZIOUTA, Y, VOLK, C, SCHMIED, B, KOCH, M, ANTOLOVIC, D
Format:	Journal Article
Language:	English
Published:	Athens Editorial Academy of the International Journal of Oncology 01-06-2006
Subjects:	Aged Biological and medical sciences CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - drug effects CD8-Positive T-Lymphocytes - immunology Dendritic Cells - immunology Female Gastroenterology. Liver. Pancreas. Abdomen Histocompatibility Antigens Class I - analysis HLA-D Antigens - analysis Humans Immunologic Memory - drug effects Interferon-gamma - pharmacology Interleukin-4 - pharmacology Liver. Biliary tract. Portal circulation. Exocrine pancreas Major Histocompatibility Complex Male Medical sciences Middle Aged Pancreatic Neoplasms - immunology Tumors Human Antigen presentation interferon-γ Helper cell Malignant tumor pancreatic cancer Cancerology Treatment Immunotherapy Pancreas cancer T-Lymphocyte Digestive diseases tumor-specific T cells Tumor cell Pancreatic disease Gamma interferon Pancreas carcinoma
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Pancreatic carcinoma is a very aggressive disease and little is known about its immunobiology. We here describe the presence in pancreatic cancer patients of spontaneously induced functional CD4 and CD8 memory/effector T cells reactive to autologous tumor cells or to the pancreatic cancer associated antigen, MUC-1. Such specific cells were present in the bone marrow or peripheral blood of most of the 23 tested patients. Low dose stimulation of primary cultures of pancreatic cancer cells with 500 IU/ml IFN-gamma for 72 h enhanced HLA-I expression and induced the de novo expression of HLA-II molecules. This led to a much better immune recognition by autologous HLA-I restricted and purified CD8 T cells and allowed tumor cell recognition by HLA-II restricted purified CD4 T-helper cells. Thus, interferon-gamma appears to be a useful adjuvant cytokine to enhance the immunogenicity of a patients' tumor cells and their recognition by tumor reactive immune cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1019-6439 1791-2423
DOI:	10.3892/ijo.28.6.1419