Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues

Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues

Nicotinamide Adenine Dinucleotide (NAD + ) plays an important role in energy metabolism and signaling pathways controlling crucial cellular functions. The increased interest in NAD + metabolism and NAD + -boosting therapies has reinforced the necessity for accurate NAD + quantification. To examine t...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 13; no. 1; pp. 2464 - 13
Main Authors: Azouaoui, Dassine, Choinière, Michael René, Khan, Momtafin, Sayfi, Shahab, Jaffer, Simran, Yousef, Selvia, Patten, David A., Green, Alexander E., Menzies, Keir J.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 11-02-2023
Nature Publishing Group
Nature Portfolio
Subjects:
631/45/320
692/53/2421
Animal tissues
Animals
Biosynthesis
Dehydrogenases
Energy Metabolism
Enzymes
Health sciences
Homeostasis
Humanities and Social Sciences
Humans
Mammals
Mammals - metabolism
Mass spectrometry
Meta-analysis
Metabolism
Metabolites
Mice
multidisciplinary
NAD
NAD - metabolism
Physiology
Rats
Science
Science (multidisciplinary)
Scientific imaging
Signal transduction
Standardization
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nicotinamide Adenine Dinucleotide (NAD + ) plays an important role in energy metabolism and signaling pathways controlling crucial cellular functions. The increased interest in NAD + metabolism and NAD + -boosting therapies has reinforced the necessity for accurate NAD + quantification. To examine the published NAD(P)(H) measures across mammalian tissues, we performed a meta-analysis of the existing data. An Ovid MEDLINE database search identified articles with NAD(P)(H) quantification results obtained from mammalian tissues published between 1961 and 2021. We screened 4890 records and extracted quantitative data, as well as the quantification methods, pre-analytical conditions, and subject characteristics. The extracted physiological NAD(P)(H) concentrations in various tissues from mice, rats, and humans, revealed an important inter- and intra-method variability that extended to recent publications. This highlights the relatively poor potential for cross-experimental analyses for NAD(P)(H) quantitative data and the importance of standardization for NAD(P)(H) quantification methods and pre-analytical procedures for future preclinical and clinical studies.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-29607-8