Immunolocalization and effect of low concentrations of Insulin like growth factor‐1 (IGF‐1) in the canine ovary

Immunolocalization and effect of low concentrations of Insulin like growth factor‐1 (IGF‐1) in the canine ovary

Insulin like growth factor‐1 (IGF‐1) plays an important role in the regulation of ovarian function. Despite its extensive study in several species, there is a paucity of information about IGF‐1`s function and localization in the canine ovary. The aim of the present study was to assess the effect of...

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Published in:Veterinary medicine and science Vol. 7; no. 1; pp. 46 - 56
Main Authors: Cardilli, Diogo J., Sousa‐Oliveira, Kellen, Franchi‐João, Carolina, Azevedo‐Voorwald, Faviana, Machado‐Silva, Marco A., Oliveira, João Ademir, Sánchez‐Calabuig, María Jesús, Toniollo, Gilson H., Pérez‐Gutiérrez, José F.
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01-01-2021
John Wiley and Sons Inc
Subjects:
bitch
Carbon dioxide
Cell growth
Corpus luteum
Fluorescence microscopy
Follicles
Granulosa cells
Growth factors
Immunohistochemistry
Insulin
insulin like growth factor‐1
Insulin-like growth factors
Kinases
Localization
maturation
oocyte
Oocytes
Original
Ovaries
Ovulation
Physiology
Reproductive status
Vagina
United Kingdom > UK
England
United States > US
insulin like growth factor-1
maturation
oocyte
bitch
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Summary:Insulin like growth factor‐1 (IGF‐1) plays an important role in the regulation of ovarian function. Despite its extensive study in several species, there is a paucity of information about IGF‐1`s function and localization in the canine ovary. The aim of the present study was to assess the effect of IGF‐1 on oocyte nuclear maturation and to immunolocalize the IGF‐1 and its receptor (IGF‐1R) in the ovary. Cumulus‐oocyte complexes (COCs) were obtained from 34 bitches. The COCs from each bitch were incubated in TCM 199‐HEPES in the absence (n = 199) or presence (n = 204) of 100 ng/ml IGF‐1 for 96 hr at 38ºC in 5% CO2, stained and evaluated for nuclear maturation by fluorescence microscopy. The results showed that the addition of IGF‐1 did not have an effect (p ˃ 0.05) on the nuclear maturation under these conditions. The immunohistochemical study revealed nuclear and cytoplasmic staining for IGF‐1 and IGF‐1R, respectively. Both were localized in all ovarian structures including the corpus luteum, but not in the granulosa cells from primordial follicles. In addition, IGF‐1 was not localized in the oocytes in tertiary follicles. The results obtained show the presence of IGF‐1 through the stages of follicular growth and in the corpus luteum of the canine ovary. However, its role on oocyte nuclear maturation could not be demonstrated. The Insulin like growth factor‐1 (IGF‐1) was localized by immunohistochemistry in the canine ovary from 34 bitches. IGF‐1 and IGF‐1R were localized in all ovarian structures including the corpus luteum, but not in the follicular cells from primordial follicles in the canine ovary. The results showed that the addition of IGF‐1 at 100 ng/ml did not have effect (p ˃ 0.05) on the nuclear maturation.
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ISSN:2053-1095
2053-1095
DOI:10.1002/vms3.347