In vivo induction of endogenous retroviruses in BALB/c mouse hepatocytes by successive treatments with carbon tetrachloride and bromodeoxyuridine

In vivo induction of endogenous retroviruses in BALB/c mouse hepatocytes by successive treatments with carbon tetrachloride and bromodeoxyuridine

The effects of bromodeoxyuridine (BrdUrd) incorporation into hepatocytes of carbon tetrachloride (CCl4)-induced regenerating liver of BALB/c mice on the induction of endogenous retroviruses was examined. From the nucliec acid hybridization studies, the maximum levels of hybridization were obtained f...

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Bibliographic Details
Published in:International journal of cancer Vol. 24; no. 4; p. 498
Main Authors: Ayukawa, H M, Saltzburg, D, Mukherjee, B B
Format: Journal Article
Language:English
Published: United States 15-10-1979
Subjects:
Animals
Bromodeoxyuridine - blood
Bromodeoxyuridine - metabolism
Bromodeoxyuridine - pharmacology
Carbon Tetrachloride - pharmacology
DNA - metabolism
DNA, Viral - biosynthesis
Liver - metabolism
Liver Regeneration - drug effects
Mice
Mice, Inbred BALB C
Nucleic Acid Hybridization
Retroviridae - drug effects
RNA - metabolism
RNA-Directed DNA Polymerase - metabolism
Virus Activation - drug effects
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Summary:The effects of bromodeoxyuridine (BrdUrd) incorporation into hepatocytes of carbon tetrachloride (CCl4)-induced regenerating liver of BALB/c mice on the induction of endogenous retroviruses was examined. From the nucliec acid hybridization studies, the maximum levels of hybridization were obtained for both N- and X-tropic BALB/c endogenous retrovirus specific [3H]-cDNAs with liver RNA from animals receiving BrdUrd at 40 and 44 h post-CCl4 treatment, and killed on the fourth day following BrdUrd injection. Media from NIH-3T3 (Swiss mouse) and mink cell cultures, infected with liver homogenates from animals treated as above, gave significant levels of reverse transcriptase activity. The observations made in the present study show that BrdUrd incorporation into cell DNA can cause induction of both N- and X-tropic endogenous retroviruses in BALB/c mouse hepatocytes in vivo, and such induction is probably a transient event.
ISSN:0020-7136
DOI:10.1002/ijc.2910240419