Histochemical and immunohistochemical investigation of guanase and nedasin in human tissues

Guanase is known as an enzyme released from the liver. Recently, cloning and sequencing of the guanase gene were reported. In addition, almost simultaneously, it was reported that an unknown protein that binds to neuronal and endocrine lethal(1)-discs large (NE-dlg), one of the membrane-associated g...

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Published in:	The Journal of Medical Investigation Vol. 53; no. 3,4; pp. 264 - 270
Main Authors:	Kubo, Kenichiro, Honda, Hirohito, Sannomiya, Katsutaka, Aying, Yuan, Mei, Wei, Mi, Shen, Aoyagi, Eriko, Simizu, Ichiro, Ii, Kunio, Ito, Susumu
Format:	Journal Article
Language:	English
Published:	Japan The University of Tokushima Faculty of Medicine 01-08-2006
Subjects:	Aminohydrolases - analysis Aminohydrolases - metabolism guanase Guanine Deaminase - analysis Guanine Deaminase - metabolism histochemical staining Histocytochemistry - methods Humans immunohistochemical staining Immunohistochemistry - methods Intestine, Small - cytology Intestine, Small - enzymology Isoenzymes - metabolism Kidney - cytology Kidney - enzymology Liver - cytology Liver - enzymology Muscle, Skeletal - cytology Muscle, Skeletal - enzymology nedasin
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Summary:	Guanase is known as an enzyme released from the liver. Recently, cloning and sequencing of the guanase gene were reported. In addition, almost simultaneously, it was reported that an unknown protein that binds to neuronal and endocrine lethal(1)-discs large (NE-dlg), one of the membrane-associated guanylate kinase homologues (MAGUK) family proteins involved in synaptic connection between neurons, was cloned and named nedasin (NE-dlg associated protein), whose sequence was almost identical to that of guanase. We immunostained fresh frozen sections of surgically removed human liver, kidney, and small intestine with anti-nedasin antibody, and simultaneously performed histochemical staining for guanase for comparison. Histochemically, guanase activity was observed in the cytoplasm of hepatocytes and biliary epithelium on the liver, in the mucosal epithelium on the small intestine, and in the proximal tubule on the kidney. Immunohistochemically, a brown discoloration due to DAB oxidation was seen in the cytoplasm of hepatocytes and biliary epithelium on the liver, in the proximal tubule but in the distal tubule a little on the kidney, in the mucosal epithelium on the small intestine. The stained region of the liver and the small intestine were different from that of the kidney. The different staining properties dependent on the organs were considered to be due to different isozymes. The physiological significance of guanase may vary with the isozymes, further studies are considered necessary. J. Med. Invest. 53: 264-270, August, 2006
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1343-1420 1349-6867
DOI:	10.2152/jmi.53.264